The protofilament substructure of amyloid fibrils

Louise C. Serpell, Margaret Sunde, Merrill D. Benson, Glenys A. Tennent, Mark B. Pepys, Paul E. Fraser

Research output: Contribution to journalArticle

273 Scopus citations

Abstract

Tissue deposition of normally soluble proteins, or their fragments, as insoluble amyloid fibrils causes the usually fatal, acquired and hereditary systemic amyloidoses and is associated with the pathology of Alzheimer's disease, type 2 diabetes and the transmissible spongiform encephalopathies. Although each type of amyloidosis is characterised by a specific amyloid fibril protein, the deposits share pathognomonic histochemical properties and the structural morphology of all amyloid fibrils is very similar. We have previously demonstrated that transthyretin amyloid fibrils contain four constituent protofilaments packed in a square array. Here, we have used cross-correlation techniques to average electron microscopy images of multiple cross-sections in order to reconstruct the substructure of ex vivo amyloid fibrils composed of amyloid A protein, monoclonal immunoglobulin λ light chain, Leu60Arg variant apolipoprotein AI, and Asp67His variant lysozyme, as well as synthetic fibrils derived from a ten-residue peptide corresponding to the A-strand of transthyretin. All the fibrils had an electron-lucent core but the packing arrangement comprised five or six protofilaments rather than four. The structural similarity that defines amyloid fibres thus exists principally at the level of β-sheet folding of the polypeptides within the protofilament, while the different types vary in the supramolecular assembly of their protofilaments. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)1033-1039
Number of pages7
JournalJournal of molecular biology
Volume300
Issue number5
DOIs
StatePublished - Jul 28 2000

Keywords

  • Amyloid
  • Electron microscopy
  • Fibrils
  • Image analysis
  • Protofilaments

ASJC Scopus subject areas

  • Virology

Fingerprint Dive into the research topics of 'The protofilament substructure of amyloid fibrils'. Together they form a unique fingerprint.

  • Cite this

    Serpell, L. C., Sunde, M., Benson, M. D., Tennent, G. A., Pepys, M. B., & Fraser, P. E. (2000). The protofilament substructure of amyloid fibrils. Journal of molecular biology, 300(5), 1033-1039. https://doi.org/10.1006/jmbi.2000.3908