The radiosensitivity of human keratinocytes: Influence of activated c-h-ras oncogene expression and tumorigenicity

Marc Mendonca, P. Boukamp, E. J. Stanbridge, J. L. Redpath

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

We have investigated the γray sensitivity of several activated c-H-ras (EJ) containing clones that have been established after transfection of the spontaneously immortalized non-tumorigenic human keratinocyte cell line HaCaT. The clones were grouped according to their tumorigenic potential after subcutaneous injection into nude mice, and fell into three classes: Class I clones A-4 and I-6 are non-tumorigenic and express very low levels of c-H-ras mRNA and no mutated ras protein (p21); Class II clones I-5 and I-7 grow to large (benign) epidermal cysts, express intermediate to high c-H-ras mRNA and variable levels of mutated ras p21 protein with clone I-5 expressing little and clone I-7 expressing high levels of p21; Class III clones II-3 and II-4 grow to solid squamous cell carcinomas, express high c-H-ras mRNA and high level of mutated p21 ras protein similar to clone I-7. Comparison of the single-hit multitarget or linear-quadratic survival curve parameters, and survival at 2 Gy (S2) indicate that there appears to be no general correlation with either activated c-H-ras expression level or tumorigenic potential, and increased radioresistance.

Original languageEnglish (US)
Pages (from-to)1195-1206
Number of pages12
JournalInternational Journal of Radiation Biology
Volume59
Issue number5
DOIs
StatePublished - 1991
Externally publishedYes

Fingerprint

oncogenes
ras Genes
Radiation Tolerance
keratinocytes
radiation tolerance
Keratinocytes
Clone Cells
clones
Proteins
ras Proteins
proteins
cysts
Cells
Messenger RNA
cultured cells
mice
Proto-Oncogene Proteins p21(ras)
rays
Epidermal Cyst
radiation resistance

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology
  • Agricultural and Biological Sciences (miscellaneous)
  • Nuclear Energy and Engineering
  • Radiation

Cite this

The radiosensitivity of human keratinocytes : Influence of activated c-h-ras oncogene expression and tumorigenicity. / Mendonca, Marc; Boukamp, P.; Stanbridge, E. J.; Redpath, J. L.

In: International Journal of Radiation Biology, Vol. 59, No. 5, 1991, p. 1195-1206.

Research output: Contribution to journalArticle

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