The rate of phagocytosis of group B streptococci and chemiluminescence in human neutrophils

M. J. Kowolik, C. G. Cumming

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Luminol-dependent neutrophil chemiluminescence (CL) is a phenomenon associated with the interrelated pathways of oxidative metabolism, and thus represents a nonspecific response to membrane stimulation. It is, however, extremely sensitive and has been correlated with microbicidal activity. The purpose of this study was to compare human neutrophil CL to the rate of phagocytosis of standard strains of group B streptococci (GBS). The GBS strains used were: Ia (090R), Ib (H36B), Ic (NCTC 11078), II (NCTC 11079), III (NCTC 11080). Phagocytosis was determined after incubating equal numbers of neutrophils and opsonised bacteria, and this was compared to the peak CL (in millivolts) obtained from challenging cells with GBS. A significant correlation (r = 0.991, p < 0.01) was demonstrated between the rate of phagocytosis and peak CL values. This is consistent with the view that both processes depend on membrane activation. Furthermore, the data support the value of developing CL assays for use in clinical neutrophil function tests.

Original languageEnglish (US)
Number of pages1
JournalIRCS Medical Science
Issue number3
StatePublished - Jan 1 1983
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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