The reinforcing effects of acetaldehyde in the posterior ventral tegmental area of alcohol-preferring rats

Zachary Rodd, Roberto I. Melendez, Alejandro Zaffaroni, Avram Goldstein, William J. McBride, Ting Kai Li

Research output: Contribution to journalArticle

120 Citations (Scopus)

Abstract

Acetaldehyde (ACD), the first metabolite of ethanol, is a biologically active compound, which may mediate some of the reinforcing, behavioral and neurotoxic effects of ethanol. The objective of this study was to test the hypothesis that ACD is reinforcing within the mesolimbic system. The intracranial self-administration (ICSA) technique was employed to determine whether ACD was reinforcing in the posterior ventral tegmental area (VTA), a site that supports the reinforcing actions of ethanol. Adult female alcohol-preferring (P) rats were implanted with guide cannulae aimed at the posterior VTA. Subjects were placed in two-lever operant chambers 7-10 days after surgery. Responding on the "active lever" on a fixed ratio 1 (FR1) schedule of reinforcement caused the delivery of 100 nl of infusate, whereas responses on the "inactive lever" were without consequences. Rats were assigned to one of five groups that self-administered either artificial cerebrospinal fluid (aCSF) throughout all eight sessions (4 h in duration) or 3- and 6-, 11- and 23-, 45- and 90- or 180- and 360-μM ACD for the eight sessions, with the lower concentration of ACD given for the initial four sessions and the higher concentration of ACD given for the last four sessions. A second experiment examined the acquisition (first four sessions), extinction (aCSF in sessions 5 and 6) and reinstatement using 90-μM ACD. A third experiment examined the effects of extending the time-out period (from 5 to 55 s) on the number and pattern of infusions of 23-μM ACD. Adult P rats readily self-administered 6-90-μM ACD and discriminated between the active and inactive levers. Furthermore, rats self-administering 90-μM ACD also demonstrated extinction behavior when aCSF was substituted for ACD and gradually reinstated active lever responding when ACD was reintroduced. P rats maintained similar numbers of infusions and infusion patterns under both time-out schedules. Overall, the data indicate that ACD is a potent reinforcer within the posterior VTA of the P rat.

Original languageEnglish
Pages (from-to)55-64
Number of pages10
JournalPharmacology Biochemistry and Behavior
Volume72
Issue number1-2
DOIs
StatePublished - 2002

Fingerprint

Ventral Tegmental Area
Acetaldehyde
Rats
Alcohols
Cerebrospinal fluid
Cerebrospinal Fluid
Ethanol
Reinforcement Schedule
Self Administration
Metabolites
Ambulatory Surgical Procedures
Surgery
Appointments and Schedules
Reinforcement

Keywords

  • Acetaldehyde
  • Alcohol
  • Alcohol-preferring rats
  • Ethanol
  • Intracranial self-administration
  • Reinforcement
  • Ventral tegmental area

ASJC Scopus subject areas

  • Biochemistry
  • Behavioral Neuroscience
  • Pharmacology

Cite this

The reinforcing effects of acetaldehyde in the posterior ventral tegmental area of alcohol-preferring rats. / Rodd, Zachary; Melendez, Roberto I.; Zaffaroni, Alejandro; Goldstein, Avram; McBride, William J.; Li, Ting Kai.

In: Pharmacology Biochemistry and Behavior, Vol. 72, No. 1-2, 2002, p. 55-64.

Research output: Contribution to journalArticle

Rodd, Zachary ; Melendez, Roberto I. ; Zaffaroni, Alejandro ; Goldstein, Avram ; McBride, William J. ; Li, Ting Kai. / The reinforcing effects of acetaldehyde in the posterior ventral tegmental area of alcohol-preferring rats. In: Pharmacology Biochemistry and Behavior. 2002 ; Vol. 72, No. 1-2. pp. 55-64.
@article{215ed0cac10f4647b11ff07d4719effb,
title = "The reinforcing effects of acetaldehyde in the posterior ventral tegmental area of alcohol-preferring rats",
abstract = "Acetaldehyde (ACD), the first metabolite of ethanol, is a biologically active compound, which may mediate some of the reinforcing, behavioral and neurotoxic effects of ethanol. The objective of this study was to test the hypothesis that ACD is reinforcing within the mesolimbic system. The intracranial self-administration (ICSA) technique was employed to determine whether ACD was reinforcing in the posterior ventral tegmental area (VTA), a site that supports the reinforcing actions of ethanol. Adult female alcohol-preferring (P) rats were implanted with guide cannulae aimed at the posterior VTA. Subjects were placed in two-lever operant chambers 7-10 days after surgery. Responding on the {"}active lever{"} on a fixed ratio 1 (FR1) schedule of reinforcement caused the delivery of 100 nl of infusate, whereas responses on the {"}inactive lever{"} were without consequences. Rats were assigned to one of five groups that self-administered either artificial cerebrospinal fluid (aCSF) throughout all eight sessions (4 h in duration) or 3- and 6-, 11- and 23-, 45- and 90- or 180- and 360-μM ACD for the eight sessions, with the lower concentration of ACD given for the initial four sessions and the higher concentration of ACD given for the last four sessions. A second experiment examined the acquisition (first four sessions), extinction (aCSF in sessions 5 and 6) and reinstatement using 90-μM ACD. A third experiment examined the effects of extending the time-out period (from 5 to 55 s) on the number and pattern of infusions of 23-μM ACD. Adult P rats readily self-administered 6-90-μM ACD and discriminated between the active and inactive levers. Furthermore, rats self-administering 90-μM ACD also demonstrated extinction behavior when aCSF was substituted for ACD and gradually reinstated active lever responding when ACD was reintroduced. P rats maintained similar numbers of infusions and infusion patterns under both time-out schedules. Overall, the data indicate that ACD is a potent reinforcer within the posterior VTA of the P rat.",
keywords = "Acetaldehyde, Alcohol, Alcohol-preferring rats, Ethanol, Intracranial self-administration, Reinforcement, Ventral tegmental area",
author = "Zachary Rodd and Melendez, {Roberto I.} and Alejandro Zaffaroni and Avram Goldstein and McBride, {William J.} and Li, {Ting Kai}",
year = "2002",
doi = "10.1016/S0091-3057(01)00733-X",
language = "English",
volume = "72",
pages = "55--64",
journal = "Pharmacology, Biochemistry and Behavior",
issn = "0091-3057",
publisher = "Elsevier Inc.",
number = "1-2",

}

TY - JOUR

T1 - The reinforcing effects of acetaldehyde in the posterior ventral tegmental area of alcohol-preferring rats

AU - Rodd, Zachary

AU - Melendez, Roberto I.

AU - Zaffaroni, Alejandro

AU - Goldstein, Avram

AU - McBride, William J.

AU - Li, Ting Kai

PY - 2002

Y1 - 2002

N2 - Acetaldehyde (ACD), the first metabolite of ethanol, is a biologically active compound, which may mediate some of the reinforcing, behavioral and neurotoxic effects of ethanol. The objective of this study was to test the hypothesis that ACD is reinforcing within the mesolimbic system. The intracranial self-administration (ICSA) technique was employed to determine whether ACD was reinforcing in the posterior ventral tegmental area (VTA), a site that supports the reinforcing actions of ethanol. Adult female alcohol-preferring (P) rats were implanted with guide cannulae aimed at the posterior VTA. Subjects were placed in two-lever operant chambers 7-10 days after surgery. Responding on the "active lever" on a fixed ratio 1 (FR1) schedule of reinforcement caused the delivery of 100 nl of infusate, whereas responses on the "inactive lever" were without consequences. Rats were assigned to one of five groups that self-administered either artificial cerebrospinal fluid (aCSF) throughout all eight sessions (4 h in duration) or 3- and 6-, 11- and 23-, 45- and 90- or 180- and 360-μM ACD for the eight sessions, with the lower concentration of ACD given for the initial four sessions and the higher concentration of ACD given for the last four sessions. A second experiment examined the acquisition (first four sessions), extinction (aCSF in sessions 5 and 6) and reinstatement using 90-μM ACD. A third experiment examined the effects of extending the time-out period (from 5 to 55 s) on the number and pattern of infusions of 23-μM ACD. Adult P rats readily self-administered 6-90-μM ACD and discriminated between the active and inactive levers. Furthermore, rats self-administering 90-μM ACD also demonstrated extinction behavior when aCSF was substituted for ACD and gradually reinstated active lever responding when ACD was reintroduced. P rats maintained similar numbers of infusions and infusion patterns under both time-out schedules. Overall, the data indicate that ACD is a potent reinforcer within the posterior VTA of the P rat.

AB - Acetaldehyde (ACD), the first metabolite of ethanol, is a biologically active compound, which may mediate some of the reinforcing, behavioral and neurotoxic effects of ethanol. The objective of this study was to test the hypothesis that ACD is reinforcing within the mesolimbic system. The intracranial self-administration (ICSA) technique was employed to determine whether ACD was reinforcing in the posterior ventral tegmental area (VTA), a site that supports the reinforcing actions of ethanol. Adult female alcohol-preferring (P) rats were implanted with guide cannulae aimed at the posterior VTA. Subjects were placed in two-lever operant chambers 7-10 days after surgery. Responding on the "active lever" on a fixed ratio 1 (FR1) schedule of reinforcement caused the delivery of 100 nl of infusate, whereas responses on the "inactive lever" were without consequences. Rats were assigned to one of five groups that self-administered either artificial cerebrospinal fluid (aCSF) throughout all eight sessions (4 h in duration) or 3- and 6-, 11- and 23-, 45- and 90- or 180- and 360-μM ACD for the eight sessions, with the lower concentration of ACD given for the initial four sessions and the higher concentration of ACD given for the last four sessions. A second experiment examined the acquisition (first four sessions), extinction (aCSF in sessions 5 and 6) and reinstatement using 90-μM ACD. A third experiment examined the effects of extending the time-out period (from 5 to 55 s) on the number and pattern of infusions of 23-μM ACD. Adult P rats readily self-administered 6-90-μM ACD and discriminated between the active and inactive levers. Furthermore, rats self-administering 90-μM ACD also demonstrated extinction behavior when aCSF was substituted for ACD and gradually reinstated active lever responding when ACD was reintroduced. P rats maintained similar numbers of infusions and infusion patterns under both time-out schedules. Overall, the data indicate that ACD is a potent reinforcer within the posterior VTA of the P rat.

KW - Acetaldehyde

KW - Alcohol

KW - Alcohol-preferring rats

KW - Ethanol

KW - Intracranial self-administration

KW - Reinforcement

KW - Ventral tegmental area

UR - http://www.scopus.com/inward/record.url?scp=0036210032&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036210032&partnerID=8YFLogxK

U2 - 10.1016/S0091-3057(01)00733-X

DO - 10.1016/S0091-3057(01)00733-X

M3 - Article

C2 - 11900769

AN - SCOPUS:0036210032

VL - 72

SP - 55

EP - 64

JO - Pharmacology, Biochemistry and Behavior

JF - Pharmacology, Biochemistry and Behavior

SN - 0091-3057

IS - 1-2

ER -