The Role of Adult Bone Marrow-Derived Stem Cells in Choroidal Neovascularization

Nilanjana Sengupta, Sergio Caballero, Robert N. Mames, Jason M. Butler, Edward W. Scott, Maria B. Grant

Research output: Contribution to journalArticle

137 Citations (Scopus)

Abstract

PURPOSE. Age-related macular degeneration (ARMD) is the primary cause of blindness in people aged of 50 years or more. The wet form leads to severe loss of central vision. Recent evidence supports that adult hematopoietic stem cells (HSCs) contribute to preretinal neovascularization. In the current study, it was determined whether HSCs, by producing both blood and blood vessels, provide functional hemangioblast activity during choroidal neovascularization (CNV) in mice. METHODS. Gfp chimeric mice were developed by bone marrow ablation of C57BL/6J mice and reconstitution with donor tissue from gfp+/+ transgenic mice. Gfp chimeric mice underwent laser rupture of Bruch's membrane and were killed and eyes enucleated at 1, 2, 3, and 4 weeks after laser injury. CNV was examined by confocal microscopy of retinal flatmounts. Because endothelial progenitor cells (EPCs) derive from HSCs, immunocytochemistry was used to quantify relative the EPC contribution to CNV. RESULTS. Laser injury alone was sufficient to induce stem cell recruitment and subsequent CNV. Gfp+ cells formed part of the functional vasculature in the choroid as early as 1 week after injury and were present for the duration of the study. The relative EPC contribution to CNV remained fairly constant throughout the study and constituted almost 50% of the total vasculature. CONCLUSIONS. Adult stem cells are recruited to the choroid in a model of CNV, where they contribute to forming aberrant new vessels. This observation suggests that targeting stem cell recruitment to the eye may offer a novel therapeutic strategy for ARMD.

Original languageEnglish (US)
Pages (from-to)4908-4913
Number of pages6
JournalInvestigative Ophthalmology and Visual Science
Volume44
Issue number11
DOIs
StatePublished - Nov 2003
Externally publishedYes

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Choroidal Neovascularization
Stem Cells
Bone Marrow
Hematopoietic Stem Cells
Adult Stem Cells
Lasers
Choroid
Macular Degeneration
Wounds and Injuries
Hemangioblasts
Bruch Membrane
Blindness
Inbred C57BL Mouse
Confocal Microscopy
Transgenic Mice
Blood Vessels
Rupture
Immunohistochemistry
Tissue Donors
Endothelial Progenitor Cells

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Sengupta, N., Caballero, S., Mames, R. N., Butler, J. M., Scott, E. W., & Grant, M. B. (2003). The Role of Adult Bone Marrow-Derived Stem Cells in Choroidal Neovascularization. Investigative Ophthalmology and Visual Science, 44(11), 4908-4913. https://doi.org/10.1167/iovs.03-0342

The Role of Adult Bone Marrow-Derived Stem Cells in Choroidal Neovascularization. / Sengupta, Nilanjana; Caballero, Sergio; Mames, Robert N.; Butler, Jason M.; Scott, Edward W.; Grant, Maria B.

In: Investigative Ophthalmology and Visual Science, Vol. 44, No. 11, 11.2003, p. 4908-4913.

Research output: Contribution to journalArticle

Sengupta, N, Caballero, S, Mames, RN, Butler, JM, Scott, EW & Grant, MB 2003, 'The Role of Adult Bone Marrow-Derived Stem Cells in Choroidal Neovascularization', Investigative Ophthalmology and Visual Science, vol. 44, no. 11, pp. 4908-4913. https://doi.org/10.1167/iovs.03-0342
Sengupta, Nilanjana ; Caballero, Sergio ; Mames, Robert N. ; Butler, Jason M. ; Scott, Edward W. ; Grant, Maria B. / The Role of Adult Bone Marrow-Derived Stem Cells in Choroidal Neovascularization. In: Investigative Ophthalmology and Visual Science. 2003 ; Vol. 44, No. 11. pp. 4908-4913.
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