The role of C. elegans Ena/VASP homolog UNC-34 in neuronal polarity and motility

Tinya Fleming, Shih Chieh Chien, Pamela J. Vanderzalm, Megan Dell, Megan K. Gavin, Wayne Forrester, Gian Garriga

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Ena/VASP proteins mediate the effects of guidance cues on the actin cytoskeleton. The single C. elegans homolog of the Ena/VASP family of proteins, UNC-34, is required for the migrations of cells and growth cones. Here we show that unc-34 mutant alleles also interact genetically with Wnt mutants to reveal a role for unc-34 in the establishment of neuronal polarity along the C. elegans anterior-posterior axis. Our mutant analysis shows that eliminating UNC-34 function results in neuronal migration and polarity phenotypes that are enhanced at higher temperatures, revealing a heat-sensitive process that is normally masked by the presence of UNC-34. Finally, we show that the UNC-34 protein is expressed broadly and accumulates in axons and at the apical junctions of epithelial cells. While most mutants lacked detectable UNC-34, three unc-34 mutants that contained missense mutations in the EVH1 domain produced full-length UNC-34 that failed to localize to apical junctions and axons, supporting the role for the EVH1 domain in localizing Ena/VASP family members.

Original languageEnglish
Pages (from-to)94-106
Number of pages13
JournalDevelopmental Biology
Volume344
Issue number1
DOIs
StatePublished - Aug 1 2010

Fingerprint

Axons
Growth Cones
Missense Mutation
Actin Cytoskeleton
Cell Movement
Cues
Hot Temperature
Epithelial Cells
Alleles
Phenotype
Temperature
Proteins
vasodilator-stimulated phosphoprotein
ENA-VASP proteins

Keywords

  • Axon guidance
  • C. elegans
  • Cell migration
  • Neuronal polarity
  • UNC-34

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology
  • Molecular Biology

Cite this

Fleming, T., Chien, S. C., Vanderzalm, P. J., Dell, M., Gavin, M. K., Forrester, W., & Garriga, G. (2010). The role of C. elegans Ena/VASP homolog UNC-34 in neuronal polarity and motility. Developmental Biology, 344(1), 94-106. https://doi.org/10.1016/j.ydbio.2010.04.025

The role of C. elegans Ena/VASP homolog UNC-34 in neuronal polarity and motility. / Fleming, Tinya; Chien, Shih Chieh; Vanderzalm, Pamela J.; Dell, Megan; Gavin, Megan K.; Forrester, Wayne; Garriga, Gian.

In: Developmental Biology, Vol. 344, No. 1, 01.08.2010, p. 94-106.

Research output: Contribution to journalArticle

Fleming, T, Chien, SC, Vanderzalm, PJ, Dell, M, Gavin, MK, Forrester, W & Garriga, G 2010, 'The role of C. elegans Ena/VASP homolog UNC-34 in neuronal polarity and motility', Developmental Biology, vol. 344, no. 1, pp. 94-106. https://doi.org/10.1016/j.ydbio.2010.04.025
Fleming, Tinya ; Chien, Shih Chieh ; Vanderzalm, Pamela J. ; Dell, Megan ; Gavin, Megan K. ; Forrester, Wayne ; Garriga, Gian. / The role of C. elegans Ena/VASP homolog UNC-34 in neuronal polarity and motility. In: Developmental Biology. 2010 ; Vol. 344, No. 1. pp. 94-106.
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