The role of DNA synthesis in peptidoglycan-induced generation of immunoglobulin-secreting cells in mice and humans

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Abstract

The requirement for DNA synthesis in peptidoglycan (PG)-induced activation of polyclonal antibodies has been studied. Inhibition of DNA synthesis with mitomycin C or hydroxyurea at the initiation of the cultures inhibited generation of over 90% of IgM- and IgG-secreting cells in PG-stimulated mouse splenocytes and of over 99.9% of IgM-, IgG- and IgA-secreting cells in PG-stimulated human peripheral blood lymphocytes. Inhibition of DNA synthesis 2 to 4 days after initiation of PG-stimulated cultures (in both mice and humans) caused an immediate decline in the numbers of Ig-secreting cells. These results demonstrate that the magnitude of PG-induced polyclonal antibody response depends on continued DNA synthesis and proliferation of Ig-secreting cells, and indicate that IgM-, IgG- and IgA-secreting cells in polyclonal activation may be actively cycling cells.

Original languageEnglish (US)
Pages (from-to)161-165
Number of pages5
JournalImmunology Letters
Volume9
Issue number2-3
DOIs
StatePublished - 1985

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Keywords

  • B cell differentiation
  • DNA synthesis
  • peptidoglycan
  • polyclonal activation

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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