The role of follicular helper T cells and the germinal center in HIV-1 gp120 DNA prime and gp120 protein boost vaccination

Kristin Hollister, Yuxin Chen, Shixia Wang, Hao Wu, Arpita Mondal, Ninah Clegg, Shan Lu, Alexander Dent

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The importance of follicular T helper (TFH) cells and the germinal center (GC) reaction in the humoral immune response has become clear in recent years, however the role of TFH cells and the GC in an HIV vaccine strategy remains unclear. In this study, we primed mice with gp120-encoding DNA and boosted with gp120 protein, a regimen previously shown to induce high titers of high affinity and cross-reactive anti-gp120 Abs. Priming with gp120 DNA caused increased TFH cell differentiation, GC B cells, and antigen-specific antibody titers, compared with priming with gp120 protein. Priming with DNA also caused more activated CD4+ T cells to become TFH cells and more GC B cells to become memory cells. Deletion of BCL6 midway through the vaccine regimen resulted in loss of TFH cells and GCs, and, unexpectedly, increased anti-gp120 IgG titers and avidity. Our data suggests vaccination with gp120-encoding DNA elicits a stronger and more rapid TFH and GC response than gp120 protein. Furthermore, we demonstrate that the GC reaction may actually limit antigen-specific IgG secretion in the context of repeated immunizations.

Original languageEnglish
Pages (from-to)1985-1992
Number of pages8
JournalHuman vaccines & immunotherapeutics
Volume10
Issue number7
DOIs
StatePublished - Jul 1 2014

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Germinal Center
Helper-Inducer T-Lymphocytes
HIV-1
Vaccination
DNA
Proteins
B-Lymphocytes
Antigens
AIDS Vaccines
Humoral Immunity
Cell Differentiation
Immunization
Vaccines
Immunoglobulin G
T-Lymphocytes
Antibodies

Keywords

  • BCL6
  • Follicular helper T cells
  • Germinal center
  • HIV vaccine
  • Prime-boost

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Pharmacology

Cite this

The role of follicular helper T cells and the germinal center in HIV-1 gp120 DNA prime and gp120 protein boost vaccination. / Hollister, Kristin; Chen, Yuxin; Wang, Shixia; Wu, Hao; Mondal, Arpita; Clegg, Ninah; Lu, Shan; Dent, Alexander.

In: Human vaccines & immunotherapeutics, Vol. 10, No. 7, 01.07.2014, p. 1985-1992.

Research output: Contribution to journalArticle

Hollister, Kristin ; Chen, Yuxin ; Wang, Shixia ; Wu, Hao ; Mondal, Arpita ; Clegg, Ninah ; Lu, Shan ; Dent, Alexander. / The role of follicular helper T cells and the germinal center in HIV-1 gp120 DNA prime and gp120 protein boost vaccination. In: Human vaccines & immunotherapeutics. 2014 ; Vol. 10, No. 7. pp. 1985-1992.
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