The role of glutamine in skeletal muscle ischemia/reperfusion injury in the rat hind limb model

Jeffrey T. Prem, Michael Eppinger, Gary Lemmon, Sidney Miller, Dan Nolan, James Peoples

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

Objectives: Ischemia/reperfusion injury is a commonly occurring event with severe pathologic consequences. Reperfusion initiates both the local and systematic damage in part through rapid oxygen generation. The glutathione system is a major mechanism of reducing this oxidative stress. If this system can be maintained or augmented during this stress then less damage may occur. Glutamine provides the source of glutamate to this system and has been shown to preserve total glutathione levels after injury/ischemia to both hepatic and gut models. To test this effect, we looked at glutamine and its role in ischemia/reperfusion injury in a rat hind limb model.Methods: Fifty male HSD/Holtzman rats weighing 350-400 g were randomized to receive glutamine (3% sol) or normal saline via intraperitoneal injections. The groups were then subjected to 2 hours of ischemia to their hind limbs using the Tourni-Cot method. Animals were then randomized to reperfusion groups of 30 minutes, 2 hours, and 4 hours. Muscle tissue assays were performed for lipid peroxidation (LPO), total glutathione (GSH), and myeloperoxidase (MPO). Peripheral blood was analyzed for creatinephosphokinase levels (CPK).Results: Animals that received glutamine showed a general trend of less lipid peroxidation products than the normal saline groups. In animals that received glutamine and underwent 2 hours of ischemia and reperfusion times of 0 minutes, 30 minutes, and 2 hours, there were significantly less percent changes in lipid peroxidation products from controls (4.6% vs 48.2%, P

Original languageEnglish (US)
Pages (from-to)147-150
Number of pages4
JournalAmerican Journal of Surgery
Volume178
Issue number2
DOIs
StatePublished - Aug 1999
Externally publishedYes

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Reperfusion Injury
Glutamine
Skeletal Muscle
Extremities
Lipid Peroxidation
Reperfusion
Glutathione
Ischemia
Polymethyl Methacrylate
Intraperitoneal Injections
Peroxidase
Sprague Dawley Rats
Glutamic Acid
Oxidative Stress
Oxygen
Muscles
Liver
Wounds and Injuries

ASJC Scopus subject areas

  • Surgery

Cite this

The role of glutamine in skeletal muscle ischemia/reperfusion injury in the rat hind limb model. / Prem, Jeffrey T.; Eppinger, Michael; Lemmon, Gary; Miller, Sidney; Nolan, Dan; Peoples, James.

In: American Journal of Surgery, Vol. 178, No. 2, 08.1999, p. 147-150.

Research output: Contribution to journalArticle

Prem, Jeffrey T. ; Eppinger, Michael ; Lemmon, Gary ; Miller, Sidney ; Nolan, Dan ; Peoples, James. / The role of glutamine in skeletal muscle ischemia/reperfusion injury in the rat hind limb model. In: American Journal of Surgery. 1999 ; Vol. 178, No. 2. pp. 147-150.
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