The role of Hex in hemangioblast and hematopoietic development.

Rebecca Chan, Robert Hromas, Mervin Yoder

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The orphan homeobox gene Hex has been shown to be critical for normal murine liver development using Hex-/- mice. Because of the early lethality of the Hex-/- mice, however, in vitro embryonic stem cell differentiation assays were employed to elucidate the role of Hex in murine hematopoiesis. These studies revealed that Hex is not required for hemangioblast development but is required in a dose-dependent fashion for hemangioblast differentiation to definitive hematopoietic progenitors. Contained here are the methods needed to conduct embryonic stem cell-derived in vitro hematopoietic assays.

Original languageEnglish
Pages (from-to)123-133
Number of pages11
JournalMethods in molecular biology (Clifton, N.J.)
Volume330
StatePublished - 2006

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Hemangioblasts
Embryonic Stem Cells
Colony-Forming Units Assay
Homeobox Genes
Hematopoiesis
Cell Differentiation
Liver
In Vitro Techniques

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

The role of Hex in hemangioblast and hematopoietic development. / Chan, Rebecca; Hromas, Robert; Yoder, Mervin.

In: Methods in molecular biology (Clifton, N.J.), Vol. 330, 2006, p. 123-133.

Research output: Contribution to journalArticle

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