The role of phosphorylation and limited proteolytic cleavage of talin and vinculin in the disruption of focal adhesion integrity

C. E. Turner, Fredrick Pavalko, K. Burridge

Research output: Contribution to journalArticle

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Abstract

Chemical agents which activate specific kinases were employed to disrupt the stress fiber and focal adhesion organization of cells spread on a substratum. The phorbol ester 12-O-tetradecanoylphorbol-13-acetate, an activator of protein kinase C, promoted a rapid loss of stress fibers and focal adhesions from African green monkey kidney (BSC-1) cells. This was paralleled by an increase in the level of talin phosphorylation suggesting that this may play a role in the removal of talin from focal adhesions. Similar morphological changes were produced in the rat embryo fibroblast line (REF 52) by dibutyryl-cAMP, which stimulates protein kinase A. In contrast, however, the phosphorylation of talin was reduced in REF 52 cells when treated with dibutyryl cAMP. In untreated cells we found that the levels of vinculin phosphorylation were very low relative to the levels of talin phosphorylation and did not change following drug treatment in either cell line. Although limited proteolytic cleavage of cytoskeletal proteins represents a potential mechanism for focal adhesion disruption, we observed no proteolysis of talin or vinculin in response to either drug treatment.

Original languageEnglish (US)
Pages (from-to)11938-11944
Number of pages7
JournalJournal of Biological Chemistry
Volume264
Issue number20
StatePublished - 1989
Externally publishedYes

Fingerprint

Talin
Vinculin
Phosphorylation
Focal Adhesions
Adhesion
Drug therapy
Stress Fibers
Proteolysis
Cercopithecus aethiops
Cytoskeletal Proteins
Fibers
Phorbol Esters
Tetradecanoylphorbol Acetate
Fibroblasts
Cyclic AMP-Dependent Protein Kinases
Pharmaceutical Preparations
Protein Kinase C
Rats
Acetates
Phosphotransferases

ASJC Scopus subject areas

  • Biochemistry

Cite this

The role of phosphorylation and limited proteolytic cleavage of talin and vinculin in the disruption of focal adhesion integrity. / Turner, C. E.; Pavalko, Fredrick; Burridge, K.

In: Journal of Biological Chemistry, Vol. 264, No. 20, 1989, p. 11938-11944.

Research output: Contribution to journalArticle

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