The role of the microtubules in tumor necrosis factor-α-induced endothelial cell permeability

Irina Petrache, Anna Birukova, Sarah I. Ramirez, Joe G N Garcia, Alexander D. Verin

Research output: Contribution to journalArticle

235 Citations (Scopus)

Abstract

Tumor necrosis factor (TNF)-α, a major proinflammatory cytokine, triggers endothelial cell activation and barrier dysfunction which are implicated in the pathogenesis of pulmonary edema associated with acute lung injury syndromes. The mechanisms of TNF-α-induced vascular permeability are not completely understood. Our initial experiments demonstrated that TNF-α-induced decreases in transendothelial electrical resistance across human pulmonary artery endothelial cells are independent of myosin light chain phosphorylation catalyzed by either myosin light chain kinase or Rho kinase. We next assessed the involvement of another cytoskeletal component, the tubulin-based microtubule network, and found TNF-α to induce a decrease in stable tubulin content and partial dissolution of peripheral microtubule network as evidenced by anti-acetylated tubulin and anti-β-tubulin immunofluorescent staining, respectively. Microtubule-stabilizing agents, paclitaxel and epothilone B, significantly attenuated TNF-α-induced decreases in transendothelial electrical resistance, inhibited the cytokine-induced increases in actin stress fibers, formation of intercellular gap, and restored the TNF-α-compromised vascular endothelial (VE)-cadherin-based cell-cell junctions. Importantly, neither TNF-α nor paclitaxel treatment was associated with endothelial cell apoptosis. Inhibition of p38 mitogen-activated protein kinase by SB203580 significantly attenuated TNF-α-induced microtubule destabilization, actin rearrangement, and endothelial barrier dysfunction. These results strongly suggest the involvement of microtubule rearrangement in TNF-α-induced endothelial cell permeability via p38 mitogen-activated protein kinase activation.

Original languageEnglish (US)
Pages (from-to)574-581
Number of pages8
JournalAmerican Journal of Respiratory Cell and Molecular Biology
Volume28
Issue number5
DOIs
StatePublished - May 1 2003
Externally publishedYes

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Endothelial cells
Microtubules
Permeability
Endothelial Cells
Tumor Necrosis Factor-alpha
Tubulin
Acoustic impedance
p38 Mitogen-Activated Protein Kinases
Paclitaxel
Electric Impedance
Actins
Chemical activation
Cytokines
Myosin-Light-Chain Kinase
rho-Associated Kinases
Stress Fibers
Myosin Light Chains
Phosphorylation
Intercellular Junctions
Acute Lung Injury

ASJC Scopus subject areas

  • Cell Biology
  • Pulmonary and Respiratory Medicine
  • Molecular Biology

Cite this

The role of the microtubules in tumor necrosis factor-α-induced endothelial cell permeability. / Petrache, Irina; Birukova, Anna; Ramirez, Sarah I.; Garcia, Joe G N; Verin, Alexander D.

In: American Journal of Respiratory Cell and Molecular Biology, Vol. 28, No. 5, 01.05.2003, p. 574-581.

Research output: Contribution to journalArticle

Petrache, Irina ; Birukova, Anna ; Ramirez, Sarah I. ; Garcia, Joe G N ; Verin, Alexander D. / The role of the microtubules in tumor necrosis factor-α-induced endothelial cell permeability. In: American Journal of Respiratory Cell and Molecular Biology. 2003 ; Vol. 28, No. 5. pp. 574-581.
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