The role of the polypyrimidine stretch at the SV40 early pre-mRNA 3' splice site in alternative splicing.

X. Y. Fu, H. Ge, J. L. Manley

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

We have studied the role in pre-mRNA splicing of the nucleotide sequence preceding the SV40 early region 3' splice site. Somewhat surprisingly, neither the pyrimidine at the highly conserved -3 position, nor the polypyrimidine stretch that extends from -5 to -15, relative to the 3' splice site, were found to be required for efficient splicing. Mutations that delete this region or create polypurine insertions at position -2 had no significant effects on the efficiency of SV40 early pre-mRNA splicing in vivo or in vitro. Interestingly, however, the pyrimidine content of this region had substantial effects on the alternative splicing pattern of this pre-mRNA in vivo. Mutations that increased the number of pyrimidine residues resulted in more efficient utilization of the large T antigen mRNA 5' splice site relative to the small t 5' splice site, while mutations that increased the purine content enhanced small t mRNA splicing. A possible molecular mechanism for these findings, as well as a model that proposes a role for the polypyrimidine stretch in alternative splicing, are discussed.

Original languageEnglish (US)
Pages (from-to)809-817
Number of pages9
JournalEMBO Journal
Volume7
Issue number3
StatePublished - Mar 1988
Externally publishedYes

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RNA Splice Sites
RNA Precursors
Alternative Splicing
Mutation
Messenger RNA
Viral Tumor Antigens
Nucleotides
pyrimidine

ASJC Scopus subject areas

  • Cell Biology
  • Genetics

Cite this

The role of the polypyrimidine stretch at the SV40 early pre-mRNA 3' splice site in alternative splicing. / Fu, X. Y.; Ge, H.; Manley, J. L.

In: EMBO Journal, Vol. 7, No. 3, 03.1988, p. 809-817.

Research output: Contribution to journalArticle

Fu, X. Y. ; Ge, H. ; Manley, J. L. / The role of the polypyrimidine stretch at the SV40 early pre-mRNA 3' splice site in alternative splicing. In: EMBO Journal. 1988 ; Vol. 7, No. 3. pp. 809-817.
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