The secY protein can act post-translationally to promote bacterial protein export

Robert Bacallao, E. Crooke, K. Shiba, W. Wickner, K. Ito

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Conditionally lethal Escherichia coli mutants in secY (prlA) show defective export of proteins to the periplasm and outer membrane. It has been proposed that this gene and other sec genes must act on pro-OmpA at an early stage of protein synthesis in order to allow later translocation to occur. We have described a temperature-sensitive mutation in which the secY(ts) function is impaired at the nonpermissive temperature (Ito, K. (1984) Mol. Gen. Genet. 197, 204-208). A plasmid bearing the wild-type secY gene under the control of the lactose operon (Shiba, K. Ito, K., Yura, T., and Cerretti, D.P. (1984) EMBO J. 3, 631-635) has been introduced into this mutant strain. We now report that the in vivo chase of pulse-labeled full length pro-OmpA to mature OmpA is accelerated by inducing the synthesis of the wild-type secY protein at the end of the period of pulse labeling. We have also assayed the requirements for secY function for in vitro protein translocation. Membranes derived from secY ts cells which were incubated at 42°C were inactive in vitro in the post-translational uptake and processing of pro-OmpA. Thus, the secY protein can act post-translationally, enhancing the translocation of completed pro-OmpA polypeptide chains across the plasma membrane.

Original languageEnglish (US)
Pages (from-to)12907-12910
Number of pages4
JournalJournal of Biological Chemistry
Volume261
Issue number27
StatePublished - 1986
Externally publishedYes

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Bacterial Proteins
Genes
Proteins
Viverridae
Bearings (structural)
Periplasm
Temperature
Lac Operon
Membranes
Protein Transport
Cell membranes
Lactose
Plasmids
Labeling
Escherichia coli
Cell Membrane
Peptides
Mutation
Processing
In Vitro Techniques

ASJC Scopus subject areas

  • Biochemistry

Cite this

The secY protein can act post-translationally to promote bacterial protein export. / Bacallao, Robert; Crooke, E.; Shiba, K.; Wickner, W.; Ito, K.

In: Journal of Biological Chemistry, Vol. 261, No. 27, 1986, p. 12907-12910.

Research output: Contribution to journalArticle

Bacallao, R, Crooke, E, Shiba, K, Wickner, W & Ito, K 1986, 'The secY protein can act post-translationally to promote bacterial protein export', Journal of Biological Chemistry, vol. 261, no. 27, pp. 12907-12910.
Bacallao, Robert ; Crooke, E. ; Shiba, K. ; Wickner, W. ; Ito, K. / The secY protein can act post-translationally to promote bacterial protein export. In: Journal of Biological Chemistry. 1986 ; Vol. 261, No. 27. pp. 12907-12910.
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