The selective enhancing influence of hemin and products of human erythrocytes on colony formation by human multipotential (CFU(GEMM)) and erythroid (BFU(E)) progenitor cells in vitro

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Abstract

The influence of erythrocytes, extracts of erythrocytes and hemin was assessed on colony formation in vitro by human bone marrow multipotential (CFU(GEMM)), erythroid (BFU(E)) and granulocyte-macrophage (CFU(GM)) progenitor cells. Colony formation by CFU(GEMM) and BFU(E) present in the low density (<1.077 g/cm3) fraction of bone marrow cells was enhanced in the presence of high density (>1.077 g/cm3) bone marrow cells. Erythrocytes, but not leukocytes, present in the high density fraction of cells were responsible for this enhancing effect on colony formation. Extracts of erythrocytes or hemin substituted for the enhancing activity of the intact erythrocytes. This enhancing activity was not apparent on colony formation by bone marrow CFU(GM). These results demonstrate a selective enhancing influence on colonies derived from CFU(GEMM) and BFU(E). Erythrocyte products allow the detection of such colonies and may play a role in the growth stimulation of CFU(GEMM) and BFU(E).

Original languageEnglish (US)
Pages (from-to)721-729
Number of pages9
JournalExperimental Hematology
Volume11
Issue number8
StatePublished - Jan 1 1983

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ASJC Scopus subject areas

  • Molecular Biology
  • Hematology
  • Genetics
  • Cell Biology
  • Cancer Research

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