The small GTPase Rap1b negatively regulates neutrophil chemotaxis and transcellular diapedesis by inhibiting Akt activation

Sachin Kumar, Juying Xu, Rupali Sani Kumar, Sribalaji Lakshmikanthan, Reuben Kapur, Matthew Kofron, Magdalena Chrzanowska-Wodnicka, Marie Dominique Filippi

Research output: Contribution to journalArticlepeer-review

37 Scopus citations

Abstract

Neutrophils are the first line of cellular defense in response to infections and inflammatory injuries. However, neutrophil activation and accumulation into tissues trigger tissue damage due to release of a plethora of toxic oxidants and proteases, a cause of acute lung injury (ALI). Despite its clinical importance, the molecular regulation of neutrophil migration is poorly understood. The small GTPase Rap1b is generally viewed as a positive regulator of immune cell functions by controlling bidirectional integrin signaling. However, we found that Rap1b-deficient mice exhibited enhanced neutrophil recruitment to inflamed lungs and enhanced susceptibility to endotoxin shock. Unexpectedly, Rap1b deficiency promoted the transcellular route of diapedesis through endothelial cell. Increased transcellular migration of Rap1b-deficient neutrophils in vitro was selectively mediated by enhanced PI3K-Akt activation and invadopodia-like protrusions. Akt inhibition in vivo suppressed excessive Rap1b-deficient neutrophil migration and associated endotoxin shock. The inhibitory action of Rap1b on PI3K signaling may be mediated by activation of phosphatase SHP-1. Thus, this study reveals an unexpected role for Rap1b as a key suppressor of neutrophil migration and lung inflammation.

Original languageEnglish (US)
Pages (from-to)1741-1758
Number of pages18
JournalJournal of Experimental Medicine
Volume211
Issue number9
DOIs
StatePublished - 2014

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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