The sperm nucleus: Chromatin, RNA, and the nuclear matrix

Graham D. Johnson, Claudia Lalancette, Amelia Linnemann, Frédéric Leduc, Guylain Boissonneault, Stephen A. Krawetz

Research output: Contribution to journalReview article

106 Citations (Scopus)

Abstract

Within the sperm nucleus, the paternal genome remains functionally inert and protected following protamination. This is marked by a structural morphogenesis that is heralded by a striking reduction in nuclear volume. Despite these changes, both human and mouse spermatozoa maintain low levels of nucleosomes that appear non-randomly distributed throughout the genome. These regions may be necessary for organizing higher order genomic structure through interactions with the nuclear matrix. The promoters of this transcriptionally quiescent genome are differentially marked by modified histones that may poise downstream epigenetic effects. This notion is supported by increasing evidence that the embryo inherits these differing levels of chromatin organization. In concert with the suite of RNAs retained in the mature sperm, they may synergistically interact to direct early embryonic gene expression. Irrespective, these features reflect the transcriptional history of spermatogenic differentiation. As such, they may soon be utilized as clinical markers of male fertility. In this review, we explore and discuss how this may be orchestrated.

Original languageEnglish (US)
Pages (from-to)21-36
Number of pages16
JournalReproduction
Volume141
Issue number1
DOIs
StatePublished - Jan 1 2011
Externally publishedYes

Fingerprint

Nuclear Matrix
Chromatin
Spermatozoa
Genome
RNA
Nucleosomes
Morphogenesis
Epigenomics
Histones
Fertility
Embryonic Structures
Biomarkers
History
Gene Expression

ASJC Scopus subject areas

  • Embryology
  • Reproductive Medicine
  • Endocrinology
  • Obstetrics and Gynecology
  • Cell Biology

Cite this

Johnson, G. D., Lalancette, C., Linnemann, A., Leduc, F., Boissonneault, G., & Krawetz, S. A. (2011). The sperm nucleus: Chromatin, RNA, and the nuclear matrix. Reproduction, 141(1), 21-36. https://doi.org/10.1530/REP-10-0322

The sperm nucleus : Chromatin, RNA, and the nuclear matrix. / Johnson, Graham D.; Lalancette, Claudia; Linnemann, Amelia; Leduc, Frédéric; Boissonneault, Guylain; Krawetz, Stephen A.

In: Reproduction, Vol. 141, No. 1, 01.01.2011, p. 21-36.

Research output: Contribution to journalReview article

Johnson, GD, Lalancette, C, Linnemann, A, Leduc, F, Boissonneault, G & Krawetz, SA 2011, 'The sperm nucleus: Chromatin, RNA, and the nuclear matrix', Reproduction, vol. 141, no. 1, pp. 21-36. https://doi.org/10.1530/REP-10-0322
Johnson GD, Lalancette C, Linnemann A, Leduc F, Boissonneault G, Krawetz SA. The sperm nucleus: Chromatin, RNA, and the nuclear matrix. Reproduction. 2011 Jan 1;141(1):21-36. https://doi.org/10.1530/REP-10-0322
Johnson, Graham D. ; Lalancette, Claudia ; Linnemann, Amelia ; Leduc, Frédéric ; Boissonneault, Guylain ; Krawetz, Stephen A. / The sperm nucleus : Chromatin, RNA, and the nuclear matrix. In: Reproduction. 2011 ; Vol. 141, No. 1. pp. 21-36.
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