The structural basis for phospholamban inhibition of the calcium pump in sarcoplasmic reticulum

Brandy L. Akin, Thomas Hurley, Zhenhui Chen, Larry Jones

Research output: Contribution to journal › Article

53 Citations (Scopus)

Abstract

Background: Phospholamban (PLB) regulates sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA) activity and is thus a key regulator of cardiac contractility. Results: We present the crystal structure of SERCA in complex with PLB at 2.8-A resolution. Conclusion: PLB stabilizes a divalent cation-free conformation of SERCA with collapsed Ca2+ binding sites. We call the structure E2-PLB. Significance: The E2-PLB structure explains how PLB decreases Ca2+ affinity and depresses cardiac contractility.

Original languageEnglish
Pages (from-to)30181-30191
Number of pages11
JournalJournal of Biological Chemistry
Volume288
Issue number42
DOIs
StatePublished - Oct 18 2013

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Sarcoplasmic Reticulum
Pumps
Calcium
Reticulum
Calcium-Transporting ATPases
Divalent Cations
Conformations
phospholamban
Crystal structure
Binding Sites

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

The structural basis for phospholamban inhibition of the calcium pump in sarcoplasmic reticulum. / Akin, Brandy L.; Hurley, Thomas; Chen, Zhenhui; Jones, Larry.

In: Journal of Biological Chemistry, Vol. 288, No. 42, 18.10.2013, p. 30181-30191.

Research output: Contribution to journal › Article

Akin, BL, Hurley, T, Chen, Z & Jones, L 2013, 'The structural basis for phospholamban inhibition of the calcium pump in sarcoplasmic reticulum', Journal of Biological Chemistry, vol. 288, no. 42, pp. 30181-30191. https://doi.org/10.1074/jbc.M113.501585
Akin BL, Hurley T, Chen Z, Jones L. The structural basis for phospholamban inhibition of the calcium pump in sarcoplasmic reticulum. Journal of Biological Chemistry. 2013 Oct 18;288(42):30181-30191. https://doi.org/10.1074/jbc.M113.501585
Akin, Brandy L. ; Hurley, Thomas ; Chen, Zhenhui ; Jones, Larry. / The structural basis for phospholamban inhibition of the calcium pump in sarcoplasmic reticulum. In: Journal of Biological Chemistry. 2013 ; Vol. 288, No. 42. pp. 30181-30191.
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