Abstract
The spore photoproduct lesion (SP; 5-thymine-5,6-dihydro-thymine) is the dominant photoproduct found in UV-irradiated spores of some bacteria such as Bacillus subtilis. Upon spore germination, this lesion is repaired in a light-independent manner by a specific repair enzyme: the spore photoproduct lyase (SP lyase). In this work, a host-guest approach in which the N-terminal fragment of Moloney murine leukemia virus reverse transcriptase (MMLV RT) serves as the host and DNA as the guest was used to determine the crystal structures of complexes including 16 bp oligo-nucleotides with and without the SP lesion at 2.14 and 1.72 Å resolution, respectively. In contrast to other types of thymine-thymine lesions, the SP lesion retains normal Watson-Crick hydrogen bonding to the adenine bases of the complementary strand, with shorter hydrogen bonds than found in the structure of the undamaged DNA. However, the lesion induces structural changes in the local conformation of what is otherwise B-form DNA. The region surrounding the lesion differs significantly in helical form from B-DNA, and the minor groove is widened by almost 3 Å compared with that of the undamaged DNA. Thus, these unusual structural features associated with SP lesions may provide a basis for recognition by the SP lyase.
| Original language | English |
|---|---|
| Pages (from-to) | 752-759 |
| Number of pages | 8 |
| Journal | Acta Crystallographica Section D: Biological Crystallography |
| Volume | 70 |
| Issue number | 3 |
| DOIs | |
| State | Published - Mar 2014 |
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Keywords
- DNA
- Host-guest approach
- Spore photoproduct
ASJC Scopus subject areas
- Structural Biology
Cite this
The structure of an authentic spore photoproduct lesion in DNA suggests a basis for recognition. / Singh, Isha; Lian, Yajun; Li, Lei; Georgiadis, Millie.
In: Acta Crystallographica Section D: Biological Crystallography, Vol. 70, No. 3, 03.2014, p. 752-759.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The structure of an authentic spore photoproduct lesion in DNA suggests a basis for recognition
AU - Singh, Isha
AU - Lian, Yajun
AU - Li, Lei
AU - Georgiadis, Millie
PY - 2014/3
Y1 - 2014/3
N2 - The spore photoproduct lesion (SP; 5-thymine-5,6-dihydro-thymine) is the dominant photoproduct found in UV-irradiated spores of some bacteria such as Bacillus subtilis. Upon spore germination, this lesion is repaired in a light-independent manner by a specific repair enzyme: the spore photoproduct lyase (SP lyase). In this work, a host-guest approach in which the N-terminal fragment of Moloney murine leukemia virus reverse transcriptase (MMLV RT) serves as the host and DNA as the guest was used to determine the crystal structures of complexes including 16 bp oligo-nucleotides with and without the SP lesion at 2.14 and 1.72 Å resolution, respectively. In contrast to other types of thymine-thymine lesions, the SP lesion retains normal Watson-Crick hydrogen bonding to the adenine bases of the complementary strand, with shorter hydrogen bonds than found in the structure of the undamaged DNA. However, the lesion induces structural changes in the local conformation of what is otherwise B-form DNA. The region surrounding the lesion differs significantly in helical form from B-DNA, and the minor groove is widened by almost 3 Å compared with that of the undamaged DNA. Thus, these unusual structural features associated with SP lesions may provide a basis for recognition by the SP lyase.
AB - The spore photoproduct lesion (SP; 5-thymine-5,6-dihydro-thymine) is the dominant photoproduct found in UV-irradiated spores of some bacteria such as Bacillus subtilis. Upon spore germination, this lesion is repaired in a light-independent manner by a specific repair enzyme: the spore photoproduct lyase (SP lyase). In this work, a host-guest approach in which the N-terminal fragment of Moloney murine leukemia virus reverse transcriptase (MMLV RT) serves as the host and DNA as the guest was used to determine the crystal structures of complexes including 16 bp oligo-nucleotides with and without the SP lesion at 2.14 and 1.72 Å resolution, respectively. In contrast to other types of thymine-thymine lesions, the SP lesion retains normal Watson-Crick hydrogen bonding to the adenine bases of the complementary strand, with shorter hydrogen bonds than found in the structure of the undamaged DNA. However, the lesion induces structural changes in the local conformation of what is otherwise B-form DNA. The region surrounding the lesion differs significantly in helical form from B-DNA, and the minor groove is widened by almost 3 Å compared with that of the undamaged DNA. Thus, these unusual structural features associated with SP lesions may provide a basis for recognition by the SP lyase.
KW - DNA
KW - Host-guest approach
KW - Spore photoproduct
UR - http://www.scopus.com/inward/record.url?scp=84896789641&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84896789641&partnerID=8YFLogxK
U2 - 10.1107/S1399004713032987
DO - 10.1107/S1399004713032987
M3 - Article
C2 - 24598744
AN - SCOPUS:84896789641
VL - 70
SP - 752
EP - 759
JO - Acta Crystallographica Section D: Structural Biology
JF - Acta Crystallographica Section D: Structural Biology
SN - 0907-4449
IS - 3
ER -