The structure of an authentic spore photoproduct lesion in DNA suggests a basis for recognition

Isha Singh, Yajun Lian, Lei Li, Millie Georgiadis

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The spore photoproduct lesion (SP; 5-thymine-5,6-dihydro-thymine) is the dominant photoproduct found in UV-irradiated spores of some bacteria such as Bacillus subtilis. Upon spore germination, this lesion is repaired in a light-independent manner by a specific repair enzyme: the spore photoproduct lyase (SP lyase). In this work, a host-guest approach in which the N-terminal fragment of Moloney murine leukemia virus reverse transcriptase (MMLV RT) serves as the host and DNA as the guest was used to determine the crystal structures of complexes including 16 bp oligo-nucleotides with and without the SP lesion at 2.14 and 1.72 Å resolution, respectively. In contrast to other types of thymine-thymine lesions, the SP lesion retains normal Watson-Crick hydrogen bonding to the adenine bases of the complementary strand, with shorter hydrogen bonds than found in the structure of the undamaged DNA. However, the lesion induces structural changes in the local conformation of what is otherwise B-form DNA. The region surrounding the lesion differs significantly in helical form from B-DNA, and the minor groove is widened by almost 3 Å compared with that of the undamaged DNA. Thus, these unusual structural features associated with SP lesions may provide a basis for recognition by the SP lyase.

Original languageEnglish
Pages (from-to)752-759
Number of pages8
JournalActa Crystallographica Section D: Biological Crystallography
Volume70
Issue number3
DOIs
StatePublished - Mar 2014

Fingerprint

Thymine
Spores
DNA
B-Form DNA
Moloney murine leukemia virus
RNA-Directed DNA Polymerase
Adenine
Hydrogen Bonding
Germination
Bacillus subtilis
Hydrogen
Nucleotides
Bacteria
Light
Enzymes
spore photoproduct lyase

Keywords

  • DNA
  • Host-guest approach
  • Spore photoproduct

ASJC Scopus subject areas

  • Structural Biology

Cite this

The structure of an authentic spore photoproduct lesion in DNA suggests a basis for recognition. / Singh, Isha; Lian, Yajun; Li, Lei; Georgiadis, Millie.

In: Acta Crystallographica Section D: Biological Crystallography, Vol. 70, No. 3, 03.2014, p. 752-759.

Research output: Contribution to journalArticle

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