The Structure of the Human Peripherin Gene (PRPH) and Identification of Potential Regulatory Elements

John Foley, Catherine A. Ley, Linda M. Parysek

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

We determined the complete nucleotide sequence of the coding region of the human peripherin gene (PRPH), as well as 742 bp 5′ to the cap site and 584 bp 3′ to the stop codon, and compared its structure and sequence to the rat and mouse genes. The overall structure of 9 exons separated by 8 introns in conserved among these three mammalian species. The nucleotide sequences of the human peripherin gene expons were 90% identical to the rat gene sequences, and the predicted human peripherin protein differed from rat peripherin at only 18 of 475 amino acid residues. Comparison of the 5′ flanking regions of the human peripherin gene and rodent genes revealed extensive areas of high homology. Additional conserved segments were found in introns 1 and 2. Within the 5′ region, potential regulatory sequences, including a nerve growth factor negative regulatory element, a Hox protein binding site, and a beat shock element, were identified in all peripherin genes. The positional conservation of each element suggests that they may be important in the tissue-specific, development-specific, and injury-specific expression of the peripherin gene.

Original languageEnglish (US)
Pages (from-to)456-461
Number of pages6
JournalGenomics
Volume22
Issue number2
DOIs
StatePublished - Jul 15 1994
Externally publishedYes

Fingerprint

Peripherins
Genes
Introns
Terminator Codon
5' Flanking Region
Nucleic Acid Regulatory Sequences
Nerve Growth Factor
Protein Binding
Exons
Rodentia
Shock
Binding Sites
Gene Expression
Amino Acids
Wounds and Injuries

ASJC Scopus subject areas

  • Genetics

Cite this

The Structure of the Human Peripherin Gene (PRPH) and Identification of Potential Regulatory Elements. / Foley, John; Ley, Catherine A.; Parysek, Linda M.

In: Genomics, Vol. 22, No. 2, 15.07.1994, p. 456-461.

Research output: Contribution to journalArticle

Foley, John ; Ley, Catherine A. ; Parysek, Linda M. / The Structure of the Human Peripherin Gene (PRPH) and Identification of Potential Regulatory Elements. In: Genomics. 1994 ; Vol. 22, No. 2. pp. 456-461.
@article{375295ba688e4595af62044193555681,
title = "The Structure of the Human Peripherin Gene (PRPH) and Identification of Potential Regulatory Elements",
abstract = "We determined the complete nucleotide sequence of the coding region of the human peripherin gene (PRPH), as well as 742 bp 5′ to the cap site and 584 bp 3′ to the stop codon, and compared its structure and sequence to the rat and mouse genes. The overall structure of 9 exons separated by 8 introns in conserved among these three mammalian species. The nucleotide sequences of the human peripherin gene expons were 90{\%} identical to the rat gene sequences, and the predicted human peripherin protein differed from rat peripherin at only 18 of 475 amino acid residues. Comparison of the 5′ flanking regions of the human peripherin gene and rodent genes revealed extensive areas of high homology. Additional conserved segments were found in introns 1 and 2. Within the 5′ region, potential regulatory sequences, including a nerve growth factor negative regulatory element, a Hox protein binding site, and a beat shock element, were identified in all peripherin genes. The positional conservation of each element suggests that they may be important in the tissue-specific, development-specific, and injury-specific expression of the peripherin gene.",
author = "John Foley and Ley, {Catherine A.} and Parysek, {Linda M.}",
year = "1994",
month = "7",
day = "15",
doi = "10.1006/geno.1994.1410",
language = "English (US)",
volume = "22",
pages = "456--461",
journal = "Genomics",
issn = "0888-7543",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - The Structure of the Human Peripherin Gene (PRPH) and Identification of Potential Regulatory Elements

AU - Foley, John

AU - Ley, Catherine A.

AU - Parysek, Linda M.

PY - 1994/7/15

Y1 - 1994/7/15

N2 - We determined the complete nucleotide sequence of the coding region of the human peripherin gene (PRPH), as well as 742 bp 5′ to the cap site and 584 bp 3′ to the stop codon, and compared its structure and sequence to the rat and mouse genes. The overall structure of 9 exons separated by 8 introns in conserved among these three mammalian species. The nucleotide sequences of the human peripherin gene expons were 90% identical to the rat gene sequences, and the predicted human peripherin protein differed from rat peripherin at only 18 of 475 amino acid residues. Comparison of the 5′ flanking regions of the human peripherin gene and rodent genes revealed extensive areas of high homology. Additional conserved segments were found in introns 1 and 2. Within the 5′ region, potential regulatory sequences, including a nerve growth factor negative regulatory element, a Hox protein binding site, and a beat shock element, were identified in all peripherin genes. The positional conservation of each element suggests that they may be important in the tissue-specific, development-specific, and injury-specific expression of the peripherin gene.

AB - We determined the complete nucleotide sequence of the coding region of the human peripherin gene (PRPH), as well as 742 bp 5′ to the cap site and 584 bp 3′ to the stop codon, and compared its structure and sequence to the rat and mouse genes. The overall structure of 9 exons separated by 8 introns in conserved among these three mammalian species. The nucleotide sequences of the human peripherin gene expons were 90% identical to the rat gene sequences, and the predicted human peripherin protein differed from rat peripherin at only 18 of 475 amino acid residues. Comparison of the 5′ flanking regions of the human peripherin gene and rodent genes revealed extensive areas of high homology. Additional conserved segments were found in introns 1 and 2. Within the 5′ region, potential regulatory sequences, including a nerve growth factor negative regulatory element, a Hox protein binding site, and a beat shock element, were identified in all peripherin genes. The positional conservation of each element suggests that they may be important in the tissue-specific, development-specific, and injury-specific expression of the peripherin gene.

UR - http://www.scopus.com/inward/record.url?scp=0028067403&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028067403&partnerID=8YFLogxK

U2 - 10.1006/geno.1994.1410

DO - 10.1006/geno.1994.1410

M3 - Article

VL - 22

SP - 456

EP - 461

JO - Genomics

JF - Genomics

SN - 0888-7543

IS - 2

ER -