The subcellular localization of yeast glycogen synthase is dependent upon glycogen content

Wayne A. Wilson, Michael P. Boyer, Keri D. Davis, Michael Burke, Peter Roach

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The budding yeast, Saccharomyces cerevisiae, accumulates the storage polysaccharide glycogen in response to nutrient limitation. Glycogen synthase, the major form of which is encoded by the GSY2 gene, catalyzes the key regulated step in glycogen storage. Here, we utilized Gsy2p fusions to green fluorescent protein (GFP) to determine where glycogen synthase was located within cells. We demonstrated that the localization pattern of Gsy2-GFP depended upon the glycogen content of the cell. When glycogen was abundant, Gsy2-GFP was found uniformly throughout the cytoplasm, but under low glycogen conditions, Gsy2-GFP localized to discrete spots within cells. Gsy2p is known to bind to glycogen, and we propose that the subcellular distribution of Gsy2-GFP reflects the distribution of glycogen particles. In the absence of glycogen, Gsy2p translocates into the nucleus. We hypothesize that Gsy2p is normally retained in the cytoplasm through its interaction with glycogen particles. When glycogen levels are reduced, Gsy2p loses this anchor and can traffic into the nucleus.

Original languageEnglish
Pages (from-to)408-420
Number of pages13
JournalCanadian Journal of Microbiology
Volume56
Issue number5
DOIs
StatePublished - May 2010

Fingerprint

Glycogen Synthase
Glycogen
Yeasts
Green Fluorescent Proteins
Cytoplasm
Saccharomycetales
Polysaccharides
Saccharomyces cerevisiae
Food

Keywords

  • Glycogen synthase
  • Localization
  • Nucleus
  • Saccharomyces cerevisiae

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Microbiology
  • Immunology
  • Genetics
  • Molecular Biology
  • Medicine(all)

Cite this

The subcellular localization of yeast glycogen synthase is dependent upon glycogen content. / Wilson, Wayne A.; Boyer, Michael P.; Davis, Keri D.; Burke, Michael; Roach, Peter.

In: Canadian Journal of Microbiology, Vol. 56, No. 5, 05.2010, p. 408-420.

Research output: Contribution to journalArticle

Wilson, Wayne A. ; Boyer, Michael P. ; Davis, Keri D. ; Burke, Michael ; Roach, Peter. / The subcellular localization of yeast glycogen synthase is dependent upon glycogen content. In: Canadian Journal of Microbiology. 2010 ; Vol. 56, No. 5. pp. 408-420.
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