The sympathetic nervous system regulates skeletal muscle motor innervation and acetylcholine receptor stability

Anna C.Z. Rodrigues, Maria L. Messi, Zhong Min Wang, Martin C. Abba, Andrea Pereyra, Alexander Birbrair, Tan Zhang, Meaghan O’Meara, Ping Kwan, Elsa I.S. Lopez, Monte Willis, Akiva Mintz, D. Clark Files, Cristina Furdui, Ronald W. Oppenheim, Osvaldo Delbono

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Aim: Symptoms of autonomic failure are frequently the presentation of advanced age and neurodegenerative diseases that impair adaptation to common physiologic stressors. The aim of this work was to examine the interaction between the sympathetic and motor nervous system, the involvement of the sympathetic nervous system (SNS) in neuromuscular junction (NMJ) presynaptic motor function, the stability of postsynaptic molecular organization, and the skeletal muscle composition and function. Methods: Since muscle weakness is a symptom of diseases characterized by autonomic dysfunction, we studied the impact of regional sympathetic ablation on muscle motor innervation by using transcriptome analysis, retrograde tracing of the sympathetic outflow to the skeletal muscle, confocal and electron microscopy, NMJ transmission by electrophysiological methods, protein analysis, and state of the art microsurgical techniques, in C57BL6, MuRF1KO and Thy-1 mice. Results: We found that the SNS regulates motor nerve synaptic vesicle release, skeletal muscle transcriptome, muscle force generated by motor nerve activity, axonal neurofilament phosphorylation, myelin thickness, and myofibre subtype composition and CSA. The SNS also modulates the levels of postsynaptic membrane acetylcholine receptor by regulating the Gα i2 -Hdac4-Myogenin-MuRF1pathway, which is prevented by the overexpression of the guanine nucleotide-binding protein Gα i2 (Q205L), a constitutively active mutant G protein subunit. Conclusion: The SNS regulates NMJ transmission, maintains optimal Gα i2 expression, and prevents any increase in Hdac4, myogenin, MuRF1, and miR-206. SNS ablation leads to upregulation of MuRF1, muscle atrophy, and downregulation of postsynaptic AChR. Our findings are relevant to clinical conditions characterized by progressive decline of sympathetic innervation, such as neurodegenerative diseases and aging.

Original languageEnglish (US)
Article numbere13195
JournalActa Physiologica
Volume225
Issue number3
DOIs
StatePublished - Mar 1 2019
Externally publishedYes

Fingerprint

Sympathetic Nervous System
Cholinergic Receptors
Skeletal Muscle
Neuromuscular Junction
Myogenin
Neurodegenerative Diseases
Autonomic Nervous System Diseases
Muscles
Guanine Nucleotides
Muscular Atrophy
Intermediate Filaments
Synaptic Vesicles
Muscle Weakness
Protein Subunits
Gene Expression Profiling
Mutant Proteins
Myelin Sheath
Transcriptome
GTP-Binding Proteins
Confocal Microscopy

Keywords

  • muscle denervation
  • muscle innervation
  • neuromuscular junction
  • skeletal muscle
  • sympathetic nervous system

ASJC Scopus subject areas

  • Physiology

Cite this

Rodrigues, A. C. Z., Messi, M. L., Wang, Z. M., Abba, M. C., Pereyra, A., Birbrair, A., ... Delbono, O. (2019). The sympathetic nervous system regulates skeletal muscle motor innervation and acetylcholine receptor stability. Acta Physiologica, 225(3), [e13195]. https://doi.org/10.1111/apha.13195

The sympathetic nervous system regulates skeletal muscle motor innervation and acetylcholine receptor stability. / Rodrigues, Anna C.Z.; Messi, Maria L.; Wang, Zhong Min; Abba, Martin C.; Pereyra, Andrea; Birbrair, Alexander; Zhang, Tan; O’Meara, Meaghan; Kwan, Ping; Lopez, Elsa I.S.; Willis, Monte; Mintz, Akiva; Files, D. Clark; Furdui, Cristina; Oppenheim, Ronald W.; Delbono, Osvaldo.

In: Acta Physiologica, Vol. 225, No. 3, e13195, 01.03.2019.

Research output: Contribution to journalArticle

Rodrigues, ACZ, Messi, ML, Wang, ZM, Abba, MC, Pereyra, A, Birbrair, A, Zhang, T, O’Meara, M, Kwan, P, Lopez, EIS, Willis, M, Mintz, A, Files, DC, Furdui, C, Oppenheim, RW & Delbono, O 2019, 'The sympathetic nervous system regulates skeletal muscle motor innervation and acetylcholine receptor stability', Acta Physiologica, vol. 225, no. 3, e13195. https://doi.org/10.1111/apha.13195
Rodrigues, Anna C.Z. ; Messi, Maria L. ; Wang, Zhong Min ; Abba, Martin C. ; Pereyra, Andrea ; Birbrair, Alexander ; Zhang, Tan ; O’Meara, Meaghan ; Kwan, Ping ; Lopez, Elsa I.S. ; Willis, Monte ; Mintz, Akiva ; Files, D. Clark ; Furdui, Cristina ; Oppenheim, Ronald W. ; Delbono, Osvaldo. / The sympathetic nervous system regulates skeletal muscle motor innervation and acetylcholine receptor stability. In: Acta Physiologica. 2019 ; Vol. 225, No. 3.
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abstract = "Aim: Symptoms of autonomic failure are frequently the presentation of advanced age and neurodegenerative diseases that impair adaptation to common physiologic stressors. The aim of this work was to examine the interaction between the sympathetic and motor nervous system, the involvement of the sympathetic nervous system (SNS) in neuromuscular junction (NMJ) presynaptic motor function, the stability of postsynaptic molecular organization, and the skeletal muscle composition and function. Methods: Since muscle weakness is a symptom of diseases characterized by autonomic dysfunction, we studied the impact of regional sympathetic ablation on muscle motor innervation by using transcriptome analysis, retrograde tracing of the sympathetic outflow to the skeletal muscle, confocal and electron microscopy, NMJ transmission by electrophysiological methods, protein analysis, and state of the art microsurgical techniques, in C57BL6, MuRF1KO and Thy-1 mice. Results: We found that the SNS regulates motor nerve synaptic vesicle release, skeletal muscle transcriptome, muscle force generated by motor nerve activity, axonal neurofilament phosphorylation, myelin thickness, and myofibre subtype composition and CSA. The SNS also modulates the levels of postsynaptic membrane acetylcholine receptor by regulating the Gα i2 -Hdac4-Myogenin-MuRF1pathway, which is prevented by the overexpression of the guanine nucleotide-binding protein Gα i2 (Q205L), a constitutively active mutant G protein subunit. Conclusion: The SNS regulates NMJ transmission, maintains optimal Gα i2 expression, and prevents any increase in Hdac4, myogenin, MuRF1, and miR-206. SNS ablation leads to upregulation of MuRF1, muscle atrophy, and downregulation of postsynaptic AChR. Our findings are relevant to clinical conditions characterized by progressive decline of sympathetic innervation, such as neurodegenerative diseases and aging.",
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AU - Birbrair, Alexander

AU - Zhang, Tan

AU - O’Meara, Meaghan

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