The Ter mutation in the dead end gene causes germ cell loss and testicular germ cell tumours

Kirsten K. Youngren, Douglas Coveney, Xiaoning Peng, Chitralekha Bhattacharya, Laura S. Schmidt, Michael L. Nickerson, Bruce T. Lamb, Jian Min Deng, Richard R. Behringer, Blanche Capel, Edward M. Rubin, Joseph H. Nadeau, Angabin Matin

Research output: Contribution to journalArticle

244 Citations (Scopus)

Abstract

In mice, the Ter mutation causes primordial germ cell (PGC) loss in all genetic backgrounds. Ter is also a potent modifier of spontaneous testicular germ cell tumour (TGCT) susceptibility in the 129 family of inbred strains, and markedly increases TGCT incidence in 129-Ter/Ter males. In 129-Ter/Ter mice, some of the remaining PGCs transform into undifferentiated pluripotent embryonal carcinoma cells, and after birth differentiate into various cells and tissues that compose TGCTs. Here, we report the positional cloning of Ter, revealing a point mutation that introduces a termination codon in the mouse orthologue (Dnd1) of the zebrafish dead end (dnd) gene. PGC deficiency is corrected both with bacterial artificial chromosomes that contain Dnd1 and with a Dnd1-encoding transgene. Dnd1 is expressed in fetal gonads during the critical period when TGCTs originate. DND1 has an RNA recognition motif and is most similar to the apobec complementation factor, a component of the cytidine to uridine RNA-editing complex. These results suggest that Ter may adversely affect essential aspects of RNA biology during PGC development. DND1 is the first protein known to have an RNA recognition motif directly implicated as a heritable cause of spontaneous tumorigenesis. TGCT development in the 129-Ter mouse strain models paediatric TGCT in humans. This work will have important implications for our understanding of the genetic control of TGCT pathogenesis and PGC biology.

Original languageEnglish (US)
Pages (from-to)360-364
Number of pages5
JournalNature
Volume435
Issue number7040
DOIs
StatePublished - May 19 2005

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Germ Cells
Mutation
Genes
129 Strain Mouse
RNA Editing
Embryonal Carcinoma Stem Cells
Bacterial Artificial Chromosomes
Cytidine
Terminator Codon
Uridine
Gonads
Zebrafish
Transgenes
Point Mutation
Cell Biology
Organism Cloning
Carcinogenesis
Testicular Germ Cell Tumor
Parturition
RNA

ASJC Scopus subject areas

  • General

Cite this

Youngren, K. K., Coveney, D., Peng, X., Bhattacharya, C., Schmidt, L. S., Nickerson, M. L., ... Matin, A. (2005). The Ter mutation in the dead end gene causes germ cell loss and testicular germ cell tumours. Nature, 435(7040), 360-364. https://doi.org/10.1038/nature03595

The Ter mutation in the dead end gene causes germ cell loss and testicular germ cell tumours. / Youngren, Kirsten K.; Coveney, Douglas; Peng, Xiaoning; Bhattacharya, Chitralekha; Schmidt, Laura S.; Nickerson, Michael L.; Lamb, Bruce T.; Deng, Jian Min; Behringer, Richard R.; Capel, Blanche; Rubin, Edward M.; Nadeau, Joseph H.; Matin, Angabin.

In: Nature, Vol. 435, No. 7040, 19.05.2005, p. 360-364.

Research output: Contribution to journalArticle

Youngren, KK, Coveney, D, Peng, X, Bhattacharya, C, Schmidt, LS, Nickerson, ML, Lamb, BT, Deng, JM, Behringer, RR, Capel, B, Rubin, EM, Nadeau, JH & Matin, A 2005, 'The Ter mutation in the dead end gene causes germ cell loss and testicular germ cell tumours', Nature, vol. 435, no. 7040, pp. 360-364. https://doi.org/10.1038/nature03595
Youngren KK, Coveney D, Peng X, Bhattacharya C, Schmidt LS, Nickerson ML et al. The Ter mutation in the dead end gene causes germ cell loss and testicular germ cell tumours. Nature. 2005 May 19;435(7040):360-364. https://doi.org/10.1038/nature03595
Youngren, Kirsten K. ; Coveney, Douglas ; Peng, Xiaoning ; Bhattacharya, Chitralekha ; Schmidt, Laura S. ; Nickerson, Michael L. ; Lamb, Bruce T. ; Deng, Jian Min ; Behringer, Richard R. ; Capel, Blanche ; Rubin, Edward M. ; Nadeau, Joseph H. ; Matin, Angabin. / The Ter mutation in the dead end gene causes germ cell loss and testicular germ cell tumours. In: Nature. 2005 ; Vol. 435, No. 7040. pp. 360-364.
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abstract = "In mice, the Ter mutation causes primordial germ cell (PGC) loss in all genetic backgrounds. Ter is also a potent modifier of spontaneous testicular germ cell tumour (TGCT) susceptibility in the 129 family of inbred strains, and markedly increases TGCT incidence in 129-Ter/Ter males. In 129-Ter/Ter mice, some of the remaining PGCs transform into undifferentiated pluripotent embryonal carcinoma cells, and after birth differentiate into various cells and tissues that compose TGCTs. Here, we report the positional cloning of Ter, revealing a point mutation that introduces a termination codon in the mouse orthologue (Dnd1) of the zebrafish dead end (dnd) gene. PGC deficiency is corrected both with bacterial artificial chromosomes that contain Dnd1 and with a Dnd1-encoding transgene. Dnd1 is expressed in fetal gonads during the critical period when TGCTs originate. DND1 has an RNA recognition motif and is most similar to the apobec complementation factor, a component of the cytidine to uridine RNA-editing complex. These results suggest that Ter may adversely affect essential aspects of RNA biology during PGC development. DND1 is the first protein known to have an RNA recognition motif directly implicated as a heritable cause of spontaneous tumorigenesis. TGCT development in the 129-Ter mouse strain models paediatric TGCT in humans. This work will have important implications for our understanding of the genetic control of TGCT pathogenesis and PGC biology.",
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