The tissue source and cellular control of the apparent size of androgen binding protein (Abp), a mouse salivary protein whose electrophoretic mobility is under the control of Sex-limited saliva pattern (Ssp)

Stephen Dlouhy, Robert C. Karn

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Expression of the dominant allele, SspSat the Sex-limited saliva pattern locus in mice results in an alteration of the electrophoretic mobility of a mouse salivary protein from the F (fast) to the S (slow) type [Karn, R. C., et al. (1980). Genetics94:s52; Karn, R. C., et al. (1982). Biochem. Genet.20:493]. We now demonstrate that the protein affected binds androgen and has a basic heterodimeric structure with the subunits connected by disulfide bridging. It is produced in the submaxillary gland where the alteration takes place. The change in electrophoretic mobility from F to S appears to be primarily the result of an increase in the molecular weight of the larger subunit since the isoelectric point changes very little. We discuss the possible causes of the increase in molecular weight.

Original languageEnglish
Pages (from-to)1057-1070
Number of pages14
JournalBiochemical Genetics
Volume21
Issue number11-12
DOIs
StatePublished - Dec 1983

Fingerprint

Androgen-Binding Protein
Salivary Proteins and Peptides
Electrophoretic mobility
saliva
androgen
androgens
Saliva
binding proteins
Molecular Weight
Molecular weight
Tissue
molecular weight
protein
gender
Submandibular Gland
mice
Isoelectric Point
isoelectric point
sulfides
Disulfides

Keywords

  • androgen binding
  • heterodimeric structure
  • mouse salivary protein
  • testicular feminization
  • two-dimensional electrophoresis

ASJC Scopus subject areas

  • Genetics
  • Biochemistry

Cite this

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AU - Karn, Robert C.

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