The tissue-specific nuclear matrix protein, NMP-2, is a member of the AML/CBF/PEBP2/runt domain transcription factor family

Interactions with the osteocalcin gene promoter

Harold L. Merriman, André J. Van Wijnen, Scott Hiebert, Joseph Bidwell, Edward Fey, Jane Lian, Janet Stein, Gary S. Stein

Research output: Contribution to journalArticle

211 Citations (Scopus)

Abstract

The nuclear matrix protein, NMP-2, was originally identified as an osteoblast-specific DNA-binding complex localized exclusively to the nuclear matrix. NMP-2 was shown to recognize two binding sites, site A (nt -605 to -599) and site B (nt -441 to -435), in the rat bone-specific osteocalcin gene promoter. This study shows that the NMP-2 binding sites A and B as well as a third NMP-2 binding site (nt -135 to -130) constitute a consensus sequence, ATGCTGGT, and represent an AML-1 recognition motif. AML-1 is a member of the AML transcription factor family which is associated with acute myelogenous leukemia and binds to the sequence TGCTGGT via its DNA-binding runt domain. Electrophoretic mobility shift assays reveal that a component of NMP-2 is a member of the AML/PEBP2/ runt domain transcription factor family based on cross-competition with AML-1 consensus oligonucleotide. Limited immunoreactivity of NMP-2 with a polyclonal N-terminal AML-1 antibody and inability of the AML-1 partner protein CBF-β to form complexes with NMP-2 indicate that NMP-2 is not identical to AML-1 but represents a variant AML/PEBP2/runt domain protein. Western and Northern blots reveal the presence of multiple AML-related proteins and AML-1 transcripts in several osseous cell lines. Furthermore, our results indicate that AML family members may selectively partition between nuclear matrix and nonmatrix compartments. Because proteins that contain a runt domain are implicated in tissue-specific transcriptional regulation, our results support the concept that the nuclear matrix mediates osteoblast-specific expression of the osteocalcin gene.

Original languageEnglish
Pages (from-to)13125-13132
Number of pages8
JournalBiochemistry
Volume34
Issue number40
StatePublished - 1995

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Core Binding Factor alpha Subunits
Nuclear Matrix-Associated Proteins
Osteocalcin
Transcription Factors
Genes
Tissue
Nuclear Matrix
Binding Sites
Osteoblasts
Proteins
Electrophoretic mobility
nuclear matrix protein 2
DNA
Consensus Sequence
Oligonucleotides
Electrophoretic Mobility Shift Assay
Rats
Acute Myeloid Leukemia
Assays
Northern Blotting

ASJC Scopus subject areas

  • Biochemistry

Cite this

The tissue-specific nuclear matrix protein, NMP-2, is a member of the AML/CBF/PEBP2/runt domain transcription factor family : Interactions with the osteocalcin gene promoter. / Merriman, Harold L.; Van Wijnen, André J.; Hiebert, Scott; Bidwell, Joseph; Fey, Edward; Lian, Jane; Stein, Janet; Stein, Gary S.

In: Biochemistry, Vol. 34, No. 40, 1995, p. 13125-13132.

Research output: Contribution to journalArticle

Merriman, Harold L. ; Van Wijnen, André J. ; Hiebert, Scott ; Bidwell, Joseph ; Fey, Edward ; Lian, Jane ; Stein, Janet ; Stein, Gary S. / The tissue-specific nuclear matrix protein, NMP-2, is a member of the AML/CBF/PEBP2/runt domain transcription factor family : Interactions with the osteocalcin gene promoter. In: Biochemistry. 1995 ; Vol. 34, No. 40. pp. 13125-13132.
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