The TRQQKRP motif located near the C-terminus of Rac2 is essential for Rac2 biologic functions and intracellular localization

Wen Tao, Marie Dominique Filippi, Jeffrey R. Bailey, Simon J. Atkinson, Bret Connors, Andrew Evan, David A. Williams

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Rac GTPases regulate a wide variety of cellular processes including actin cytoskeleton organization, gene expression, cell-cycle progression, and apoptosis. Here we report that the TRQQKRP motif of Rac2 located near the C-terminus, a region of sequence disparity among Rac proteins, is essential for complementation of Rac2 function in Rac2-deficient cells. Deletion of this sequence can also intragenically suppress the dominant-negative Rac2D57N mutation in a variety of functional assays. In Rac2-deficient cells, expression of TRQQKRP-deleted Rac2 protein is unable to completely rescue migration and nicotinamide adenine dinucleotide phosphate oxidase deficiencies previously described in these cells. In fibroblasts, the Rac2D57N mutant phenotypes of abnormal proliferation, cell morphology, and membrane ruffling are suppressed by the TRQQKRP motif deletion. In myeloid hematopoietic cells, the deletion of the TRQQKRP motif eliminates a Rac2D57N- induced block in in vitro differentiation of neutrophils not previously described with this mutant. Mechanistically, deletion of the TRQQKRP motif results in diminished geranylgeranylation and delocalization of intracellular Rac2 protein. Taken together, these results indicate that the TRQQKRP motif in Rac2 protein is required for efficient prenylation and correct intracellular localization of Rac2 protein and is essential for Rac2 to mediate a variety of its biologic functions. These data suggest that precise localization of Rac2 protein in intracellular compartments and/or with other proteins/lipids is a prerequisite for its diverse functions.

Original languageEnglish
Pages (from-to)1679-1688
Number of pages10
JournalBlood
Volume100
Issue number5
StatePublished - Sep 1 2002

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Prenylation
Proteins
rac GTP-Binding Proteins
Sequence Deletion
GTP Phosphohydrolases
Cell proliferation
Myeloid Cells
Fibroblasts
Actin Cytoskeleton
NADP
Gene expression
Actins
Assays
Cell Cycle
Oxidoreductases
Neutrophils
Cells
Cell Membrane
Apoptosis
Membranes

ASJC Scopus subject areas

  • Hematology

Cite this

Tao, W., Filippi, M. D., Bailey, J. R., Atkinson, S. J., Connors, B., Evan, A., & Williams, D. A. (2002). The TRQQKRP motif located near the C-terminus of Rac2 is essential for Rac2 biologic functions and intracellular localization. Blood, 100(5), 1679-1688.

The TRQQKRP motif located near the C-terminus of Rac2 is essential for Rac2 biologic functions and intracellular localization. / Tao, Wen; Filippi, Marie Dominique; Bailey, Jeffrey R.; Atkinson, Simon J.; Connors, Bret; Evan, Andrew; Williams, David A.

In: Blood, Vol. 100, No. 5, 01.09.2002, p. 1679-1688.

Research output: Contribution to journalArticle

Tao, W, Filippi, MD, Bailey, JR, Atkinson, SJ, Connors, B, Evan, A & Williams, DA 2002, 'The TRQQKRP motif located near the C-terminus of Rac2 is essential for Rac2 biologic functions and intracellular localization', Blood, vol. 100, no. 5, pp. 1679-1688.
Tao W, Filippi MD, Bailey JR, Atkinson SJ, Connors B, Evan A et al. The TRQQKRP motif located near the C-terminus of Rac2 is essential for Rac2 biologic functions and intracellular localization. Blood. 2002 Sep 1;100(5):1679-1688.
Tao, Wen ; Filippi, Marie Dominique ; Bailey, Jeffrey R. ; Atkinson, Simon J. ; Connors, Bret ; Evan, Andrew ; Williams, David A. / The TRQQKRP motif located near the C-terminus of Rac2 is essential for Rac2 biologic functions and intracellular localization. In: Blood. 2002 ; Vol. 100, No. 5. pp. 1679-1688.
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