The Tryptophan Bridge Is a Critical Feature of the Papillomavirus E2 DNA Binding Domain

Karen Corina, Steven R. Grossman, James Barsoum, S. S. Prakash, Elliot Androphy, R. Blake Pepinsky

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The papillomavirus E2 protein is a DNA binding protein that regulates viral transcription and replication. E2 binds DNA as a dimer. Recent crystallographic data for E2 complexed to DNA revealed that novel peptide structures in E2 mediated dimerization and DNA binding. To identify important features of these motifs we have used limited proteolysis and urea denaturation as biochemical probes for structure, applying these techniques to E2 alone, E2 bound to DNA, cross-linked products, and mutants that were targeted at Trp360, a contact point along the dimer interface. DNA binding stabilized E2 structure, shifting the point at which it denatures from 5 to 7.6 M urea. In contrast, Trp360 mutant proteins, while dimeric, were more sensitive to denaturation by urea when bound to DNA. The most striking results came from uv cross-linking studies in which Trp360 was targeted as the site of cross-linking. Ultraviolet cross-linking dramatically increased the resistance of E2 to proteolysis regardless of the protease tested and with no deleterious effect on the affinity of E2 for DNA. Cross-linking through Cys356 with bismaleimidohexane did not promote stabilization. The ability to stabilize or destabilize E2 by Trp360-targeted modifications demonstrates the importance of the Trp360-Trp360 interaction, which may represent a general feature of the β-barrel motif.

Original languageEnglish (US)
Pages (from-to)391-396
Number of pages6
JournalVirology
Volume197
Issue number1
DOIs
StatePublished - Nov 1993
Externally publishedYes

Fingerprint

Tryptophan
DNA
Urea
Proteolysis
DNA-Binding Proteins
Dimerization
Mutant Proteins
Peptide Hydrolases
Peptides
Proteins

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology

Cite this

Corina, K., Grossman, S. R., Barsoum, J., Prakash, S. S., Androphy, E., & Pepinsky, R. B. (1993). The Tryptophan Bridge Is a Critical Feature of the Papillomavirus E2 DNA Binding Domain. Virology, 197(1), 391-396. https://doi.org/10.1006/viro.1993.1600

The Tryptophan Bridge Is a Critical Feature of the Papillomavirus E2 DNA Binding Domain. / Corina, Karen; Grossman, Steven R.; Barsoum, James; Prakash, S. S.; Androphy, Elliot; Pepinsky, R. Blake.

In: Virology, Vol. 197, No. 1, 11.1993, p. 391-396.

Research output: Contribution to journalArticle

Corina, K, Grossman, SR, Barsoum, J, Prakash, SS, Androphy, E & Pepinsky, RB 1993, 'The Tryptophan Bridge Is a Critical Feature of the Papillomavirus E2 DNA Binding Domain', Virology, vol. 197, no. 1, pp. 391-396. https://doi.org/10.1006/viro.1993.1600
Corina, Karen ; Grossman, Steven R. ; Barsoum, James ; Prakash, S. S. ; Androphy, Elliot ; Pepinsky, R. Blake. / The Tryptophan Bridge Is a Critical Feature of the Papillomavirus E2 DNA Binding Domain. In: Virology. 1993 ; Vol. 197, No. 1. pp. 391-396.
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