The ubiquitin ligase SCFGrrl is necessary for pheromone sensitivity in Saccharomyces cerevisiae

Kelly Schweitzer, Ross Cocklin, Lisa Garrett, Falguni Desai, Mark Goebl

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The presence of the appropriate pheromone induces α and a cells of the yeast Saccharomyces cerevisiae to activate both changes in transcriptional expression and cell polarity that eventually lead to the mating of α and a cells to form a/α diploid cells. A third response after exposure to mating pheromone is a transient cell cycle arrest, allowing synchronization of the two cell types in G1 prior to cell fusion. At least in part, this cell cycle arrest requires the inactivation of Cln-kinase activity through transcriptional inactivation of the CLN1 and CLN2 genes, degradation of the Cln proteins and direct inhibition of Cln-kinase complexes. Here we report that GRR1, which encodes a substrate recognition subunit of SCF complexes, is critical for pheromone sensitivity and likely for this arrest. Loss of SCFGrr1 function by deletion of the GRR1 gene causes pheromone resistance. However, deletion of CLN1 and CLN2 restores pheromone sensitivity to grr1Δ cells. Thus, rapid loss of Cln-kinase activity during mating may require coordinated inactivation of the Cln-kinase complexes, inactivation of CLN transcription and SCFGrr1-dependent Cln degradation.

Original languageEnglish
Pages (from-to)553-564
Number of pages12
JournalYeast
Volume22
Issue number7
DOIs
StatePublished - May 2005

Fingerprint

Pheromones
Ligases
ubiquitin
Ubiquitin
ligases
Yeast
pheromones
Saccharomyces cerevisiae
Genes
Cells
inactivation
phosphotransferases (kinases)
Degradation
Phosphotransferases
Transcription
Synchronization
Cell Cycle Checkpoints
Fusion reactions
cells
Proteins

Keywords

  • Cell division cycle
  • F-box protein
  • Mating pathway
  • SCF
  • Ubiquitin

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Bioengineering
  • Microbiology

Cite this

The ubiquitin ligase SCFGrrl is necessary for pheromone sensitivity in Saccharomyces cerevisiae. / Schweitzer, Kelly; Cocklin, Ross; Garrett, Lisa; Desai, Falguni; Goebl, Mark.

In: Yeast, Vol. 22, No. 7, 05.2005, p. 553-564.

Research output: Contribution to journalArticle

Schweitzer, Kelly ; Cocklin, Ross ; Garrett, Lisa ; Desai, Falguni ; Goebl, Mark. / The ubiquitin ligase SCFGrrl is necessary for pheromone sensitivity in Saccharomyces cerevisiae. In: Yeast. 2005 ; Vol. 22, No. 7. pp. 553-564.
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