The ubiquitin ligase SCFGrrl is necessary for pheromone sensitivity in Saccharomyces cerevisiae

Kelly Schweitzer, Ross Cocklin, Lisa Garrett, Falguni Desai, Mark Goebl

Research output: Contribution to journalArticle

10 Scopus citations


The presence of the appropriate pheromone induces α and a cells of the yeast Saccharomyces cerevisiae to activate both changes in transcriptional expression and cell polarity that eventually lead to the mating of α and a cells to form a/α diploid cells. A third response after exposure to mating pheromone is a transient cell cycle arrest, allowing synchronization of the two cell types in G1 prior to cell fusion. At least in part, this cell cycle arrest requires the inactivation of Cln-kinase activity through transcriptional inactivation of the CLN1 and CLN2 genes, degradation of the Cln proteins and direct inhibition of Cln-kinase complexes. Here we report that GRR1, which encodes a substrate recognition subunit of SCF complexes, is critical for pheromone sensitivity and likely for this arrest. Loss of SCFGrr1 function by deletion of the GRR1 gene causes pheromone resistance. However, deletion of CLN1 and CLN2 restores pheromone sensitivity to grr1Δ cells. Thus, rapid loss of Cln-kinase activity during mating may require coordinated inactivation of the Cln-kinase complexes, inactivation of CLN transcription and SCFGrr1-dependent Cln degradation.

Original languageEnglish (US)
Pages (from-to)553-564
Number of pages12
Issue number7
StatePublished - May 1 2005


  • Cell division cycle
  • F-box protein
  • Mating pathway
  • SCF
  • Ubiquitin

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Bioengineering
  • Microbiology

Fingerprint Dive into the research topics of 'The ubiquitin ligase SCF<sup>Grrl</sup> is necessary for pheromone sensitivity in Saccharomyces cerevisiae'. Together they form a unique fingerprint.

  • Cite this