The unique action for bone metabolism of 1α,25-(OH)2D3-26,23-lactone

Mamoru Kiyoki, Noriyoshi Kurihara, Seiichi Ishizuka, Shigeyuki Ishii, Yoshiyuki Hakeda, Masayoshi Kumegawa, Anthony W. Norman

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

[23(S),25(R)]-1α,25-Dihydroxyvitamin D3-26,23-lactone ([23(S),25(R)]-1α,25-(OH)2D3-26,23-lactone) increased dose-dependently alkaline phosphatase activity in osteoblastic cells, clone MC3T3-E1, in medium containing 0.1% bovine serum albumin. The maximal stimulated enzyme activity per mg protein was 1.6-fold over that of control cultures at 250 pg/ml. The metabolite also increased collagen synthesis in a dose-related fashion. On the other hand, [23(S),25(R)]-1α,25-(OH)2D3-26,23-lactone decreased slightly but significantly 45Ca mobilization, and blocked the resorptive action of 1α,25-dihydroxyvitamin D3 but not that of parathyroid hormone, in mouse calvaria in organ culture. These results indicate that [23(S),25(R)]-1α,25-(OH)2D3-26,23-lactone stimulates the differentiation of osteoblasts and inhibits bone resorption in vitro.

Original languageEnglish (US)
Pages (from-to)693-698
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume127
Issue number2
DOIs
StatePublished - Mar 15 1985
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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