The use of filtration techniques for the lysis and study of red blood cells

Donald E. Hultquist, Michael R. Vasko, Robert H. Gray

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A filtration technique for the gentle lysis of erythrocytes has been developed using cellulose triacetate membranes. When cell suspensions are filtered under nitrogen pressure, lysis occurs at the surface of the filter in such a way that the cell ghosts are retained on the filter. The contents of the cell are extruded through the pores of the filter without mixing with the cell suspension. Cell ghosts and intact erythrocytes have been collected on membranes and examined by electron microscopy. These preparations have the advantage of being free of the structural artifacts that result from centrifugation. In addition, the filter facilitates preparation for electron microscopy by providing a support for the sample during fixation and then dissolving during the dehydration of the sample.

Original languageEnglish (US)
Pages (from-to)237-244
Number of pages8
JournalAmerican Journal of Hematology
Volume3
Issue number4
DOIs
StatePublished - 1977

Fingerprint

Erythrocyte Membrane
Hemolysis
Centrifugation
Chickens
Electron Microscopy
Erythrocytes
Cell Membrane
Suspensions
Membranes
Dehydration
Artifacts
Nitrogen
Pressure

Keywords

  • cell membrane
  • electron microscopy
  • erythrocytes
  • filtration
  • hemolysis

ASJC Scopus subject areas

  • Hematology

Cite this

The use of filtration techniques for the lysis and study of red blood cells. / Hultquist, Donald E.; Vasko, Michael R.; Gray, Robert H.

In: American Journal of Hematology, Vol. 3, No. 4, 1977, p. 237-244.

Research output: Contribution to journalArticle

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