The Use of Fluorescence Redistribution After Photobleaching for Analysis of Cellular Microtubule Dynamics

Claire Walczak, Rania S. Rizk, Sidney L. Shaw

Research output: Book/ReportBook

7 Citations (Scopus)

Abstract

Microtubules (MTs) are highly dynamic polymers that serve as tracks for vesicular movement during interphase and as structural components of the mitotic spindle, which is used to segregate the genetic material. MT dynamics are highly regulated wherein MTs turnover differentially between interphase and mitosis. Within the mitotic spindle, there are distinct classes of MTs with different dynamic properties. To understand how cellular proteins regulate the dynamics of MTs, it is necessary to have methods to assess their turnover properties. In this chapter we present approaches to assess MT dynamics in cultured mammalian cells using fluorescence redistribution after photobleaching. We include a discussion of cell culture and imaging conditions that maintain cell viability. We also provide an extensive discussion of both data collection and analysis that are utilized to estimate the turnover dynamics of MTs.

Original languageEnglish
PublisherUnknown Publisher
Number of pages18
Volume97
EditionC
DOIs
StatePublished - 2010

Publication series

NameMethods in Cell Biology
No.C
Volume97
ISSN (Print)0091679X

Fingerprint

Photobleaching
Microtubules
Fluorescence
Spindle Apparatus
Interphase
Mitosis
Cultured Cells
Cell Survival
Polymers
Cell Culture Techniques

ASJC Scopus subject areas

  • Cell Biology

Cite this

Walczak, C., Rizk, R. S., & Shaw, S. L. (2010). The Use of Fluorescence Redistribution After Photobleaching for Analysis of Cellular Microtubule Dynamics. (C ed.) (Methods in Cell Biology; Vol. 97, No. C). Unknown Publisher. https://doi.org/10.1016/S0091-679X(10)97003-9

The Use of Fluorescence Redistribution After Photobleaching for Analysis of Cellular Microtubule Dynamics. / Walczak, Claire; Rizk, Rania S.; Shaw, Sidney L.

C ed. Unknown Publisher, 2010. 18 p. (Methods in Cell Biology; Vol. 97, No. C).

Research output: Book/ReportBook

Walczak, Claire ; Rizk, Rania S. ; Shaw, Sidney L. / The Use of Fluorescence Redistribution After Photobleaching for Analysis of Cellular Microtubule Dynamics. C ed. Unknown Publisher, 2010. 18 p. (Methods in Cell Biology; C).
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