The vaccinia virus N1L protein influences cytokine secretion in vitro after infection

Zhouning Zhang, Melissa Rose Abrahams, Lawrence A. Hunt, Jill Suttles, William Marshall, Debomoy Lahiri, Girish J. Kotwal

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The vaccinia virus N1L ORF encodes a protein that enhances virulence and replication of the virus by an unknown mechanism. It has been studied for its ability to enhance viral replication and dissemination in the brain and more recently has been linked to an immunomodulatory role in which it inhibits the activation of cytokine transcription activators in Toll-like receptor signaling pathways after pathogen recognition. The effect of N1L on the release of cytokines from human primary monocytes was investigated. Secretion of the proinflammatory, antiviral cytokines TNF-α, IL-1β, IFN-α, IFN-β, and the anti-inflammatory cytokine IL-10 was found to be inhibited by the presence of the N1L protein.

Original languageEnglish
Pages (from-to)69-86
Number of pages18
JournalAnnals of the New York Academy of Sciences
Volume1056
DOIs
StatePublished - 2005

Fingerprint

Cytokines
Infection
Viruses
Vaccinia virus
Toll-Like Receptors
Pathogens
Transcription
Virus Replication
Interleukin-1
Interleukin-10
Transcriptional Activation
Open Reading Frames
Antiviral Agents
Virulence
Monocytes
Brain
Proteins
Anti-Inflammatory Agents
Chemical activation
In Vitro Techniques

Keywords

  • Cytokines
  • IFN-β
  • N1L ORF
  • NF-κB
  • TNF

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

The vaccinia virus N1L protein influences cytokine secretion in vitro after infection. / Zhang, Zhouning; Abrahams, Melissa Rose; Hunt, Lawrence A.; Suttles, Jill; Marshall, William; Lahiri, Debomoy; Kotwal, Girish J.

In: Annals of the New York Academy of Sciences, Vol. 1056, 2005, p. 69-86.

Research output: Contribution to journalArticle

Zhang, Zhouning ; Abrahams, Melissa Rose ; Hunt, Lawrence A. ; Suttles, Jill ; Marshall, William ; Lahiri, Debomoy ; Kotwal, Girish J. / The vaccinia virus N1L protein influences cytokine secretion in vitro after infection. In: Annals of the New York Academy of Sciences. 2005 ; Vol. 1056. pp. 69-86.
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