The vascular endothelial growth factor receptor Flt-1 mediates biological activities. Implications for a functional role of placenta growth factor in monocyte activation and chemotaxis

Matthias Clauss, Herbert Weich, Georg Breier, Ulrike Knies, Wolfgang Röckl, Johannes Waltenberger, Werner Risaut

Research output: Contribution to journalArticle

721 Citations (Scopus)

Abstract

Two distinct receptors for vascular endothelial growth factor (VEGF), the tyrosine kinase receptors Flt-1 and Flk-1/KDR, have been described. In this study we show that monocytes, in contrast to endothelium, express only the VEGF receptor Flt-1, and that this receptor specifically binds also the VEGF homolog placenta growth factor (PIGF). Both VEGF and PIGF stimulate tissue factor production and chemotaxis in monocytes at equivalent doses. In contrast, endothelial cells expressing both the Flt-1 and the Flk-1/KDR receptors produce more tissue factor upon stimulation with VEGF than after stimulation with PIGF. Neutralizing antibodies to the KDR receptor reduce the VEGF-stimulated tissue factor induction in endothelial cells to levels obtained by stimulation with PIGF alone, but do not affect PIGF-induced tissue factor induction in endothelial cells nor the VEGF-dependent tissue factor production in monocytes. These findings strongly suggest Flt-1 as a functional receptor for VEGF and PIGF in monocytes and endothelial cells and identify this receptor as a mediator of monocyte recruitment and procoagulant activity.

Original languageEnglish (US)
Pages (from-to)17629-17634
Number of pages6
JournalJournal of Biological Chemistry
Volume271
Issue number30
DOIs
StatePublished - 1996
Externally publishedYes

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Vascular Endothelial Growth Factor Receptor-1
Vascular Endothelial Growth Factor Receptor
Thromboplastin
Chemotaxis
Bioactivity
Endothelial cells
Monocytes
Intercellular Signaling Peptides and Proteins
Chemical activation
Vascular Endothelial Growth Factor A
Endothelial Cells
Personnel Selection
Vascular Endothelial Growth Factor Receptor-2
Receptor Protein-Tyrosine Kinases
Neutralizing Antibodies
Endothelium
Placenta Growth Factor

ASJC Scopus subject areas

  • Biochemistry

Cite this

The vascular endothelial growth factor receptor Flt-1 mediates biological activities. Implications for a functional role of placenta growth factor in monocyte activation and chemotaxis. / Clauss, Matthias; Weich, Herbert; Breier, Georg; Knies, Ulrike; Röckl, Wolfgang; Waltenberger, Johannes; Risaut, Werner.

In: Journal of Biological Chemistry, Vol. 271, No. 30, 1996, p. 17629-17634.

Research output: Contribution to journalArticle

Clauss, Matthias ; Weich, Herbert ; Breier, Georg ; Knies, Ulrike ; Röckl, Wolfgang ; Waltenberger, Johannes ; Risaut, Werner. / The vascular endothelial growth factor receptor Flt-1 mediates biological activities. Implications for a functional role of placenta growth factor in monocyte activation and chemotaxis. In: Journal of Biological Chemistry. 1996 ; Vol. 271, No. 30. pp. 17629-17634.
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