The visna virus long terminal repeat directs expression of a receptor gene in activated macrophages, lymphocytes, and the central nervous systems of transgenic mice

J. A. Small, C. Bieberich, Z. Ghotbi, Jay Hess, G. A. Scangos, J. E. Clements

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Abstract

Visna virus is a lentivirus which causes a slow progressive disease involving the immune system and the central nervous system. To determine the role of the viral long terminal repeat (LTR) in targeting the virus to specific host cells and tissues, transgenic mice were constructed which contained the visna virus LTR directing expression of the bacterial gene encoding chloramphenicol acetyltransferase (CAT). Analysis of the transgenic mouse tissues for CAT activity revealed that the viral LTR was responsible, in part, for the tropism of visna virus for macrophages and the central nervous system. Expression of the LTR required the macrophage to be in an activated state both in vivo and in vitro. Thioglycolate activation of peritoneal macrophages in vivo and 12-O-tetradecanoylphorbol 13-acetate treatment in vitro induced expression of the visna virus LTR. Lymphocytes from the spleens of the transgenic mice expressed CAT activity, suggesting that visna virus was able to replicate in lymphocytes, as did human immunodeficiency virus and simian immunodeficiency virus. These studies demonstrated that the lentivirus LTR was responsible, in part, for cell and tissue tropism in vivo.

Original languageEnglish (US)
Pages (from-to)1891-1896
Number of pages6
JournalJournal of Virology
Volume63
Issue number5
StatePublished - 1989
Externally publishedYes

Fingerprint

Visna-maedi virus
Visna maedi virus
terminal repeat sequences
Terminal Repeat Sequences
Transgenic Mice
central nervous system
macrophages
lymphocytes
Central Nervous System
Macrophages
genetically modified organisms
Lymphocytes
chloramphenicol acetyltransferase
receptors
Chloramphenicol O-Acetyltransferase
mice
Genes
Lentivirus
Tropism
genes

ASJC Scopus subject areas

  • Immunology

Cite this

The visna virus long terminal repeat directs expression of a receptor gene in activated macrophages, lymphocytes, and the central nervous systems of transgenic mice. / Small, J. A.; Bieberich, C.; Ghotbi, Z.; Hess, Jay; Scangos, G. A.; Clements, J. E.

In: Journal of Virology, Vol. 63, No. 5, 1989, p. 1891-1896.

Research output: Contribution to journalArticle

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