The yeast cell cycle gene CDC34 encodes a ubiquitin-conjugating enzyme

Mark Goebl, John Yochem, Stefan Jentsch, John P. Mcgrath, Alexander Varshavsky, Breck Byers

Research output: Contribution to journalArticle

304 Citations (Scopus)

Abstract

Mutants in the gene CDC34 of the yeast Saccharomyces cerevisiae are defective in the transition from G1 to the S phase of the cell cycle. This gene was cloned and shown to encode a 295-residue protein that has substantial sequence similarity to the product of the yeast RAD6 gene. The RAD6 gene is required for a variety of cellular functions including DNA repair and was recently shown to encode a ubiquitin-conjugating enzyme. When produced in Escherichia coli, the CDC34 gene product catalyzed the covalent attachment of ubiquitin to histones H2A and H2B in vitro, demonstrating that the CDC34 protein is another distinct member of the family of ubiquitin-conjugating enzymes. The cell cycle function of CDC34 is thus likely to be mediated by the ubiquitin-conjugating activity of its product.

Original languageEnglish (US)
Pages (from-to)1331-1335
Number of pages5
JournalScience
Volume241
Issue number4871
StatePublished - 1988
Externally publishedYes

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Ubiquitin-Conjugating Enzymes
cdc Genes
Yeasts
Genes
Ubiquitin
Cell Cycle
S Phase
DNA Repair
Histones
Saccharomyces cerevisiae
Proteins
Escherichia coli

ASJC Scopus subject areas

  • General

Cite this

Goebl, M., Yochem, J., Jentsch, S., Mcgrath, J. P., Varshavsky, A., & Byers, B. (1988). The yeast cell cycle gene CDC34 encodes a ubiquitin-conjugating enzyme. Science, 241(4871), 1331-1335.

The yeast cell cycle gene CDC34 encodes a ubiquitin-conjugating enzyme. / Goebl, Mark; Yochem, John; Jentsch, Stefan; Mcgrath, John P.; Varshavsky, Alexander; Byers, Breck.

In: Science, Vol. 241, No. 4871, 1988, p. 1331-1335.

Research output: Contribution to journalArticle

Goebl, M, Yochem, J, Jentsch, S, Mcgrath, JP, Varshavsky, A & Byers, B 1988, 'The yeast cell cycle gene CDC34 encodes a ubiquitin-conjugating enzyme', Science, vol. 241, no. 4871, pp. 1331-1335.
Goebl M, Yochem J, Jentsch S, Mcgrath JP, Varshavsky A, Byers B. The yeast cell cycle gene CDC34 encodes a ubiquitin-conjugating enzyme. Science. 1988;241(4871):1331-1335.
Goebl, Mark ; Yochem, John ; Jentsch, Stefan ; Mcgrath, John P. ; Varshavsky, Alexander ; Byers, Breck. / The yeast cell cycle gene CDC34 encodes a ubiquitin-conjugating enzyme. In: Science. 1988 ; Vol. 241, No. 4871. pp. 1331-1335.
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