The yeast Hrq1 helicase stimulates Pso2 translesion nuclease activity and thereby promotes DNA interstrand crosslink repair

Cody M. Rogers, Chun Ying Lee, Samuel Parkins, Nicholas J. Buehler, Sabine Wenzel, Francisco Martínez-Márquez, Yuichiro Takagi, Sua Myong, Matthew L. Bochman

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

DNA interstrand crosslink (ICL) repair requires a complex network of DNA damage response pathways. Removal of the ICL lesions is vital, as they are physical barriers to essential DNA processes that require the separation of duplex DNA, such as replication and transcription. The Fanconi anemia (FA) pathway is the principal mechanism for ICL repair in metazoans and is coupled to DNA replication. In Saccharomyces cerevisiae, a vestigial FA pathway is present, but ICLs are predominantly repaired by a pathway involving the Pso2 nuclease, which is hypothesized to use its exonuclease activity to digest through the lesion to provide access for translesion polymerases. However, Pso2 lacks translesion nuclease activity in vitro, and mechanistic details of this pathway are lacking, especially relative to FA. We recently identified the Hrq1 helicase, a homolog of the disease-linked enzyme RecQ-like helicase 4 (RECQL4), as a component of Pso2-mediated ICL repair. Here, using genetic, biochemical, and biophysical approaches, including single-molecule FRET (smFRET)- and gel-based nuclease assays, we show that Hrq1 stimulates the Pso2 nuclease through a mechanism that requires Hrq1 catalytic activity. Importantly, Hrq1 also stimulated Pso2 translesion nuclease activity through a site-specific ICL in vitro We noted that stimulation of Pso2 nuclease activity is specific to eukaryotic RecQ4 subfamily helicases, and genetic and biochemical data suggest that Hrq1 likely interacts with Pso2 through their N-terminal domains. These results advance our understanding of FA-independent ICL repair and establish a role for the RecQ4 helicases in the repair of these detrimental DNA lesions.

Original languageEnglish (US)
Pages (from-to)8945-8957
Number of pages13
JournalThe Journal of biological chemistry
Volume295
Issue number27
DOIs
StatePublished - Jul 3 2020

Keywords

  • DNA damage
  • DNA helicase
  • DNA interstrand crosslink (ICL) repair
  • DNA repair
  • DNA-protein interaction
  • Hrq1
  • Pso2
  • RECQL4
  • SNM1A
  • Saccharomyces cerevisiae
  • helicase
  • nuclease
  • single-molecule Förster resonance energy transfer (smFRET)

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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