The yeast SRM1 protein and human RCC1 protein share analogous functions.

K. L. Clark, M. Ohtsubo, T. Nishimoto, Mark Goebl, G. F. Sprague

Research output: Contribution to journalArticle

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Abstract

The Saccharomyces cerevisiae protein SRM1 and the mammalian protein RCC1 have amino acid sequence similarity throughout their lengths. SRM1 was defined by a recessive mutation in yeast that both activates the signal transduction pathway required for mating and leads to arrest in the G1 phase of the cell cycle. RCC1 was defined by a recessive mutation in hamster cells that causes premature chromosome condensation and other characteristics of entry into mitosis. Despite the seemingly different roles implied by these phenotypes, we suggest that RCC1 and SRM1 proteins have similar functions. In particular, we find that RCC1 can complement the temperature-sensitive growth phenotype of two independent srm1 mutations and also complements, at least partially, phenotypes associated with activation of the pheromone response pathway, such as transcription induction of FUS1. However, RCC1 fails to complement an srm1 null allele. Further characterization of the srm1 mutant phenotype reveals a defect in plasmid and chromosome stability, suggesting that the mutants have a defect in DNA replication, mitosis, or their coordination. Finally, like RCC1, SRM1 is a nuclear protein. Together, these data imply that SRM1 and RCC1 have a common role in their respective organisms.

Original languageEnglish (US)
Pages (from-to)781-792
Number of pages12
JournalCell regulation
Volume2
Issue number10
StatePublished - Oct 1991

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Fungal Proteins
Phenotype
Mitosis
Mutation
Saccharomyces cerevisiae Proteins
Chromosomal Instability
Pheromones
G1 Phase
Nuclear Proteins
DNA Replication
Cricetinae
Amino Acid Sequence
Signal Transduction
Cell Cycle
Proteins
Plasmids
Chromosomes
Yeasts
Alleles
Temperature

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Clark, K. L., Ohtsubo, M., Nishimoto, T., Goebl, M., & Sprague, G. F. (1991). The yeast SRM1 protein and human RCC1 protein share analogous functions. Cell regulation, 2(10), 781-792.

The yeast SRM1 protein and human RCC1 protein share analogous functions. / Clark, K. L.; Ohtsubo, M.; Nishimoto, T.; Goebl, Mark; Sprague, G. F.

In: Cell regulation, Vol. 2, No. 10, 10.1991, p. 781-792.

Research output: Contribution to journalArticle

Clark, KL, Ohtsubo, M, Nishimoto, T, Goebl, M & Sprague, GF 1991, 'The yeast SRM1 protein and human RCC1 protein share analogous functions.', Cell regulation, vol. 2, no. 10, pp. 781-792.
Clark KL, Ohtsubo M, Nishimoto T, Goebl M, Sprague GF. The yeast SRM1 protein and human RCC1 protein share analogous functions. Cell regulation. 1991 Oct;2(10):781-792.
Clark, K. L. ; Ohtsubo, M. ; Nishimoto, T. ; Goebl, Mark ; Sprague, G. F. / The yeast SRM1 protein and human RCC1 protein share analogous functions. In: Cell regulation. 1991 ; Vol. 2, No. 10. pp. 781-792.
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