Therapeutic interventions in mice with chronic proliferative dermatitis (cpdm/cpdm)

M. J J Gijbels, G. R. Elliott, Harm HogenEsch, C. Zurcher, HovenA Van Den, P. L B Bruijnzeel

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Chronic proliferative dermatitis (cpd) is a spontaneous mutation in C57BL/Ka mice (cpdm/cpdm). The dermatitis is characterized by redness, hairloss, scaling, pruritus and histologically by epithelial hyperproliferation, infiltration of eosinophils, macrophages and mast cells. Lesions similar to those in the skin occur in the esophagus and forestomach. In this paper, we describe the effect of drug treatments directed against epidermal hyperproliferation (calcipotriene and etretinate), against inflammation (corticosteroids and dapsone) and against pruritus (loratidine and capsaicin). The criteria used to objectively estimate the effect of the treatment were 1) macroscopic evaluation of the lesions (cpd score), 2) degree of epithelial hyperproliferation assessed by BrdU incorporation and epithelial thickness, and 3) microscopic evaluation of the inflammatory cells in the skin samples. Treatment of the cpdm/cpdm mice with calcipotriene (5 μg/day for 3 weeks) inhibited epidermal proliferation and the number of eosinophils. Systemic etretinate treatment (30 μg/g/day for 3 weeks) was not very effective. Topical corticosteroids (0.05 μg/day, for 3 weeks) exerted a therapeutic effect on the hyperproliferation and the number of eosinophils. Oral dapsone treatment (34 μg/g/day, for 5 weeks) reduced the BrdU incorporation in the skin and the epithelial thickness in the esophagus. The anti-histamine loratidine (orally, 1.7 μg/g/day, for 4 weeks) reduced the severity of the lesions macroscopically, probably by suppressing the pruritus. Capsaicin (topically, 30 mM, for 5 weeks) also reduced the severity of the macroscopic observable lesions. Moreover, capsaicin reduced the dorsal and ventral epidermal thickness. The results from this and previous studies indicate that steroids (topically and systemically) and less strongly calcipotriene are the most effective treatments for the lesions observed in the cpdm/cpdm mice, since both hyperproliferation and the influx of eosinophils are reduced. Although the pathogenesis of the cpd lesions remains to be determined, our results indicate that the cpdm/cpdm mouse can be used to investigate new drugs for their possible application in chronic dermatitis.

Original languageEnglish (US)
Pages (from-to)351-358
Number of pages8
JournalExperimental Dermatology
Volume9
Issue number5
DOIs
StatePublished - 2000
Externally publishedYes

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Dermatitis
Eosinophils
Capsaicin
Pruritus
Etretinate
Dapsone
Skin
Bromodeoxyuridine
Esophagus
Adrenal Cortex Hormones
Drug therapy
Therapeutics
Macrophages
Therapeutic Uses
Inbred C57BL Mouse
Infiltration
Mast Cells
Pharmaceutical Preparations
Histamine
Steroids

Keywords

  • Eosinophil
  • Hyperproliferation
  • Mouse model
  • Skin
  • Therapy

ASJC Scopus subject areas

  • Dermatology

Cite this

Therapeutic interventions in mice with chronic proliferative dermatitis (cpdm/cpdm). / Gijbels, M. J J; Elliott, G. R.; HogenEsch, Harm; Zurcher, C.; Van Den, HovenA; Bruijnzeel, P. L B.

In: Experimental Dermatology, Vol. 9, No. 5, 2000, p. 351-358.

Research output: Contribution to journalArticle

Gijbels, M. J J ; Elliott, G. R. ; HogenEsch, Harm ; Zurcher, C. ; Van Den, HovenA ; Bruijnzeel, P. L B. / Therapeutic interventions in mice with chronic proliferative dermatitis (cpdm/cpdm). In: Experimental Dermatology. 2000 ; Vol. 9, No. 5. pp. 351-358.
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