Thermoacoustic in vivo determination of blood oxygenation

William L. Kiser, Robert A. Kruger, Daniel Reinecke, Gabe Kruger, Kathy Miller

Research output: Chapter in Book/Report/Conference proceedingConference contribution

10 Scopus citations


We have utilized a prototype Thermoacoustic Computed Tomography Small Animal Imaging System to acquire images of athymic mice with bilateral tumors implanted in the cranial mammary fat pads. The breast tumor cell lines used in the study, which are MCF7, and MCF7 transfected with Vascular Endothelial Growth Factor (VEGF), exhibit distinctly contrasting levels of vascularization. Three dimensional images of the mice, acquired using pulses of NIR stimulating light, demonstrate the ability of the system to generate high resolution images of the vascular system up to one inch deep in tissue, and at the same time, differentiate tissue types based on the infrared absorption properties of the tissue; a property related in part to blood content and oxygenation levels. We have processed images acquired at different stimulating wavelengths to generate images representative of the distribution of oxygenated and deoxygenated hemoglobin throughout the tumors. The images demonstrate the in vivo capabilities of the imaging system and map system structure as well as the total, oxygenated and deoxygenated hemoglobin components of the blood.

Original languageEnglish
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
EditorsA.A. Oraevsky, L.V. Wang
Number of pages7
StatePublished - 2004
EventPhotons Plus Ultrasound: Imaging and Sensing - San Jose, CA, United States
Duration: Jan 25 2004Jan 26 2004


OtherPhotons Plus Ultrasound: Imaging and Sensing
CountryUnited States
CitySan Jose, CA


  • Blood oxygenation
  • Thermoacoustic Computed Tomography (TCT)
  • Tumor vascularization

ASJC Scopus subject areas

  • Engineering(all)

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    Kiser, W. L., Kruger, R. A., Reinecke, D., Kruger, G., & Miller, K. (2004). Thermoacoustic in vivo determination of blood oxygenation. In A. A. Oraevsky, & L. V. Wang (Eds.), Progress in Biomedical Optics and Imaging - Proceedings of SPIE (9 ed., Vol. 5, pp. 1-7). [01]