Thermoacoustic molecular imaging of small animals

Robert A. Kruger, William L. Kiser, Daniel R. Reinecke, Gabe A. Kruger, Kathy Miller

Research output: Contribution to journalArticle

197 Citations (Scopus)

Abstract

We have designed, constructed, and tested a thermoacoustic computed tomography (TCT) scanner for imaging optical absorption in small animals in three dimensions. The device utilizes pulsed laser irradiation (680-1064 nm) and a unique, 128-element transducer array. We quantified the isotropic spatial resolution of this scanner to be 0.35 mm. We describe a dual-wavelength substraction technique for isolating optical dyes with TCT. Phantom experiments demonstrate that we can detect 5 fmol of a near-infrared dye (indocyanine green, ICG) in a 1-μL volume using dual-wavelength subtraction. Initial TCT imaging in phantoms and in two sacrificed mice suggests that three-dimensional, optical absorption patterns in small animals can be detected with an order of magnitude better spatial resolution and an order of magnitude better low-contrast detectability in small animals when compared to fluorescence imaging or diffusion optical tomography.

Original languageEnglish (US)
Pages (from-to)113-123
Number of pages11
JournalMolecular Imaging
Volume2
Issue number2
DOIs
StatePublished - Apr 2003

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Thermoacoustics
Molecular imaging
Molecular Imaging
Tomography
Animals
Optical Imaging
Imaging techniques
Light absorption
Coloring Agents
Imaging Phantoms
Dyes
Optical Tomography
X-Ray Computed Tomography Scanners
Wavelength
Indocyanine Green
Optical tomography
Laser beam effects
Transducers
Pulsed lasers
Lasers

Keywords

  • Flourescence
  • Optical imaging
  • Optoacoustics
  • Photoacoustics
  • Thermoacoustics

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Kruger, R. A., Kiser, W. L., Reinecke, D. R., Kruger, G. A., & Miller, K. (2003). Thermoacoustic molecular imaging of small animals. Molecular Imaging, 2(2), 113-123. https://doi.org/10.1162/153535003322331993

Thermoacoustic molecular imaging of small animals. / Kruger, Robert A.; Kiser, William L.; Reinecke, Daniel R.; Kruger, Gabe A.; Miller, Kathy.

In: Molecular Imaging, Vol. 2, No. 2, 04.2003, p. 113-123.

Research output: Contribution to journalArticle

Kruger, RA, Kiser, WL, Reinecke, DR, Kruger, GA & Miller, K 2003, 'Thermoacoustic molecular imaging of small animals', Molecular Imaging, vol. 2, no. 2, pp. 113-123. https://doi.org/10.1162/153535003322331993
Kruger, Robert A. ; Kiser, William L. ; Reinecke, Daniel R. ; Kruger, Gabe A. ; Miller, Kathy. / Thermoacoustic molecular imaging of small animals. In: Molecular Imaging. 2003 ; Vol. 2, No. 2. pp. 113-123.
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