Thermoregulatory, carboxyatractylate-sensitive uncoupling in heart and skeletal muscle mitochondria of the ground squirrel correlates with the level of free fatty acids

Nikolai Broustovetski, M. V. Egorova, D. Yu Gnutov, V. G. Gogvadze, E. N. Mokhova, V. P. Skulachev

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Thermoregulatory uncoupling of oxidative phosphorylation has been studied in heart and skeletal muscle mitochondria of ground squirrels. The respiratory rate of mitochondria in the presence of oligomycin was found to be much higher in winter (in hibernating, arousing, or aroused animals) than in summer. This additional respiration is strongly (arousing animals) or completely (hibernating and aroused animals) inhibited by carboxy-atractylate (CAtr) and bovine serum albumin (BSA). The CAtr- and BSA-induced decreases in the rate of respiration are accompanied by membrane potential increases. The rate of the CAtr- and BSA-sensitive respiration is proportional to the content of free fatty acids which, in the heart, decreases in the order: arousing > aroused = hibernating > summer animals. Maximal respiratory rates observed in the presence of dinitrophenol (arousing > aroused > summer > hibernating animals) do not parallel the fatty acid level. It is assumed that some heat production in the winter animals is due to fatty acid-induced, ATPIADP-antiporter-mediated uncoupling in heart and skeletal muscle mitochondria. The peak of heat production during arousal after hibernation also includes some other stimulatory effect on mitochondrial respiration.

Original languageEnglish (US)
Pages (from-to)15-17
Number of pages3
JournalFEBS Letters
Volume305
Issue number1
DOIs
StatePublished - Jun 22 1992
Externally publishedYes

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Muscle Mitochondrion
Sciuridae
Mitochondria
Nonesterified Fatty Acids
Muscle
Myocardium
Animals
Skeletal Muscle
Atractyloside
Respiratory Rate
Bovine Serum Albumin
Respiration
Thermogenesis
Fatty Acids
Dinitrophenols
Antiporters
Oligomycins
Hibernation
Oxidative Phosphorylation
Arousal

Keywords

  • Fatty acid
  • Hibernation
  • Thermoregulatory uncoupling

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Thermoregulatory, carboxyatractylate-sensitive uncoupling in heart and skeletal muscle mitochondria of the ground squirrel correlates with the level of free fatty acids. / Broustovetski, Nikolai; Egorova, M. V.; Gnutov, D. Yu; Gogvadze, V. G.; Mokhova, E. N.; Skulachev, V. P.

In: FEBS Letters, Vol. 305, No. 1, 22.06.1992, p. 15-17.

Research output: Contribution to journalArticle

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