Abstract
Thermoregulatory uncoupling of oxidative phosphorylation has been studied in heart and skeletal muscle mitochondria of ground squirrels. The respiratory rate of mitochondria in the presence of oligomycin was found to be much higher in winter (in hibernating, arousing, or aroused animals) than in summer. This additional respiration is strongly (arousing animals) or completely (hibernating and aroused animals) inhibited by carboxy-atractylate (CAtr) and bovine serum albumin (BSA). The CAtr- and BSA-induced decreases in the rate of respiration are accompanied by membrane potential increases. The rate of the CAtr- and BSA-sensitive respiration is proportional to the content of free fatty acids which, in the heart, decreases in the order: arousing > aroused = hibernating > summer animals. Maximal respiratory rates observed in the presence of dinitrophenol (arousing > aroused > summer > hibernating animals) do not parallel the fatty acid level. It is assumed that some heat production in the winter animals is due to fatty acid-induced, ATPIADP-antiporter-mediated uncoupling in heart and skeletal muscle mitochondria. The peak of heat production during arousal after hibernation also includes some other stimulatory effect on mitochondrial respiration.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 15-17 |
| Number of pages | 3 |
| Journal | FEBS Letters |
| Volume | 305 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jun 22 1992 |
| Externally published | Yes |
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Keywords
- Fatty acid
- Hibernation
- Thermoregulatory uncoupling
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Thermoregulatory, carboxyatractylate-sensitive uncoupling in heart and skeletal muscle mitochondria of the ground squirrel correlates with the level of free fatty acids. / Broustovetski, Nikolai; Egorova, M. V.; Gnutov, D. Yu; Gogvadze, V. G.; Mokhova, E. N.; Skulachev, V. P.
In: FEBS Letters, Vol. 305, No. 1, 22.06.1992, p. 15-17.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Thermoregulatory, carboxyatractylate-sensitive uncoupling in heart and skeletal muscle mitochondria of the ground squirrel correlates with the level of free fatty acids
AU - Broustovetski, Nikolai
AU - Egorova, M. V.
AU - Gnutov, D. Yu
AU - Gogvadze, V. G.
AU - Mokhova, E. N.
AU - Skulachev, V. P.
PY - 1992/6/22
Y1 - 1992/6/22
N2 - Thermoregulatory uncoupling of oxidative phosphorylation has been studied in heart and skeletal muscle mitochondria of ground squirrels. The respiratory rate of mitochondria in the presence of oligomycin was found to be much higher in winter (in hibernating, arousing, or aroused animals) than in summer. This additional respiration is strongly (arousing animals) or completely (hibernating and aroused animals) inhibited by carboxy-atractylate (CAtr) and bovine serum albumin (BSA). The CAtr- and BSA-induced decreases in the rate of respiration are accompanied by membrane potential increases. The rate of the CAtr- and BSA-sensitive respiration is proportional to the content of free fatty acids which, in the heart, decreases in the order: arousing > aroused = hibernating > summer animals. Maximal respiratory rates observed in the presence of dinitrophenol (arousing > aroused > summer > hibernating animals) do not parallel the fatty acid level. It is assumed that some heat production in the winter animals is due to fatty acid-induced, ATPIADP-antiporter-mediated uncoupling in heart and skeletal muscle mitochondria. The peak of heat production during arousal after hibernation also includes some other stimulatory effect on mitochondrial respiration.
AB - Thermoregulatory uncoupling of oxidative phosphorylation has been studied in heart and skeletal muscle mitochondria of ground squirrels. The respiratory rate of mitochondria in the presence of oligomycin was found to be much higher in winter (in hibernating, arousing, or aroused animals) than in summer. This additional respiration is strongly (arousing animals) or completely (hibernating and aroused animals) inhibited by carboxy-atractylate (CAtr) and bovine serum albumin (BSA). The CAtr- and BSA-induced decreases in the rate of respiration are accompanied by membrane potential increases. The rate of the CAtr- and BSA-sensitive respiration is proportional to the content of free fatty acids which, in the heart, decreases in the order: arousing > aroused = hibernating > summer animals. Maximal respiratory rates observed in the presence of dinitrophenol (arousing > aroused > summer > hibernating animals) do not parallel the fatty acid level. It is assumed that some heat production in the winter animals is due to fatty acid-induced, ATPIADP-antiporter-mediated uncoupling in heart and skeletal muscle mitochondria. The peak of heat production during arousal after hibernation also includes some other stimulatory effect on mitochondrial respiration.
KW - Fatty acid
KW - Hibernation
KW - Thermoregulatory uncoupling
UR - http://www.scopus.com/inward/record.url?scp=0026741066&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026741066&partnerID=8YFLogxK
U2 - 10.1016/0014-5793(92)80645-W
DO - 10.1016/0014-5793(92)80645-W
M3 - Article
VL - 305
SP - 15
EP - 17
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 1
ER -