Three-dimensional configuration of crypts of different types of colorectal adenomas

K. Araki, T. Ogata, G. M. Roomans, W. H. Wilborn, Andrew Evan, T. Ushiki, K. A. Warfel

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The three-dimensional configuration of isolated crypts of normal human colonic mucosa and colorectal adenomas was examined by scanning electron microscopy. For isolation of the crypts, the digestion method with HCl was used for formalin fixed tissues, and the separation method with ethylenediaminetetraacetic acid (EDTA) following ultrasonication was applied to fresh tissues. In a comparative study, the NaOH cell-maceration method, which visualized the sub-basal laminal collagen sheath, was applied. The isolated crypts from the normal colon were visualized as a single straight tubule resembling a test tube. Most isolated crypts of the tubular adenomas were visualized as elongated fan-like structures with several protuberances and a few short branchings. Their average length was more than twice that of the normal colonic mucosa crypts. Most crypts of the villous adenomas were visualized as slender tubules without protuberances and short branchings, and their average length was three times that of the tubular adenoma crypts. Most crypts of the tubulovillous adenomas were long and triangular with several longitudinal folds and protuberances, and the average length was about three times that of the tubular adenoma crypts.

Original languageEnglish (US)
Pages (from-to)149-158
Number of pages10
JournalScanning Microscopy
Volume9
Issue number1
StatePublished - 1995
Externally publishedYes

Fingerprint

protuberances
Tissue
Ethylenediaminetetraacetic acid
configurations
Collagen
Formaldehyde
Fans
ethylenediaminetetraacetic acids
collagens
fans
sheaths
Scanning electron microscopy
isolation
tubes
scanning electron microscopy
cells
Mucous Membrane

Keywords

  • colonic adenoma
  • crypt isolation
  • ethylene-diaminetetraacetic acid (EDTA)
  • Scanning electron microscopy
  • three-dimension
  • tissue digestion

ASJC Scopus subject areas

  • Instrumentation

Cite this

Araki, K., Ogata, T., Roomans, G. M., Wilborn, W. H., Evan, A., Ushiki, T., & Warfel, K. A. (1995). Three-dimensional configuration of crypts of different types of colorectal adenomas. Scanning Microscopy, 9(1), 149-158.

Three-dimensional configuration of crypts of different types of colorectal adenomas. / Araki, K.; Ogata, T.; Roomans, G. M.; Wilborn, W. H.; Evan, Andrew; Ushiki, T.; Warfel, K. A.

In: Scanning Microscopy, Vol. 9, No. 1, 1995, p. 149-158.

Research output: Contribution to journalArticle

Araki, K, Ogata, T, Roomans, GM, Wilborn, WH, Evan, A, Ushiki, T & Warfel, KA 1995, 'Three-dimensional configuration of crypts of different types of colorectal adenomas', Scanning Microscopy, vol. 9, no. 1, pp. 149-158.
Araki K, Ogata T, Roomans GM, Wilborn WH, Evan A, Ushiki T et al. Three-dimensional configuration of crypts of different types of colorectal adenomas. Scanning Microscopy. 1995;9(1):149-158.
Araki, K. ; Ogata, T. ; Roomans, G. M. ; Wilborn, W. H. ; Evan, Andrew ; Ushiki, T. ; Warfel, K. A. / Three-dimensional configuration of crypts of different types of colorectal adenomas. In: Scanning Microscopy. 1995 ; Vol. 9, No. 1. pp. 149-158.
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