Three-dimensional engineered bone-ligament-bone constructs for anterior cruciate ligament replacement

Jinjin Ma, Michael J. Smietana, Tatiana Kostrominova, Edward M. Wojtys, Lisa M. Larkin, Ellen M. Arruda

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

The anterior cruciate ligament (ACL), a major stabilizer of the knee, is commonly injured. Because of its intrinsic poor healing ability, a torn ACL is usually reconstructed by a graft. We developed a multi-phasic, or bone-ligament-bone, tissue-engineered construct for ACL grafts using bone marrow stromal cells and sheep as a model system. After 6 months in vivo, the constructs increased in cross section and exhibited a well-organized microstructure, native bone integration, a functional enthesis, vascularization, innervation, increased collagen content, and structural alignment. The constructs increased in stiffness to 52% of the tangent modulus and 95% of the geometric stiffness of native ACL. The viscoelastic response of the explants was virtually indistinguishable from that of adult ACL. These results suggest that our constructs after implantation can obtain physiologically relevant structural and functional characteristics comparable to those of adult ACL. They present a viable option for ACL replacement.

Original languageEnglish (US)
Pages (from-to)103-116
Number of pages14
JournalTissue Engineering - Part A
Volume18
Issue number1-2
DOIs
StatePublished - Jan 1 2012

Fingerprint

Anterior Cruciate Ligament
Ligaments
Bone
Bone and Bones
Grafts
Transplants
Stiffness
Mesenchymal Stromal Cells
Collagen
Knee
Sheep
Tissue
Microstructure

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Biomedical Engineering
  • Biomaterials
  • Medicine(all)

Cite this

Three-dimensional engineered bone-ligament-bone constructs for anterior cruciate ligament replacement. / Ma, Jinjin; Smietana, Michael J.; Kostrominova, Tatiana; Wojtys, Edward M.; Larkin, Lisa M.; Arruda, Ellen M.

In: Tissue Engineering - Part A, Vol. 18, No. 1-2, 01.01.2012, p. 103-116.

Research output: Contribution to journalArticle

Ma, Jinjin ; Smietana, Michael J. ; Kostrominova, Tatiana ; Wojtys, Edward M. ; Larkin, Lisa M. ; Arruda, Ellen M. / Three-dimensional engineered bone-ligament-bone constructs for anterior cruciate ligament replacement. In: Tissue Engineering - Part A. 2012 ; Vol. 18, No. 1-2. pp. 103-116.
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