Three-dimensional imaging of human skin and mucosa by two-photon laser scanning microscopy

Janine C. Malone, Antoinette F. Hood, Tameka Conley, Jens Nürnberger, Lee Ann Baldridge, Jeffrey L. Clendenon, Kenneth Dunn, Carrie Phillips

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Background: Various structural components of human skin biopsy specimens are difficult to visualize using conventional histologic approaches. Methods: We used two-photon microscopy and advanced imaging software to render three-dimensional (3D) images of in situ nerves, blood vessels, and hair follicles labeled with various fluorescent markers. Archived frozen human skin biopsy specimens were cryosectioned up to 150 μm in thickness and fluorescently stained with rhodamine- or fluorescein-labeled antibodies or lectins. Optical sections were collected by two-photon microscopy and the resulting data sets were analyzed in three dimensions using Voxx software. Results: Reconstructed image volumes demonstrated the complex 3D morphology of nerves, blood vessels and adnexal structures in normal mucocutaneous tissue. Conclusion: Two-photon microscopy and Voxx rendering software allow for detailed 3D visualization of structures within human mucocutaneous biopsy specimens, as they appear in situ, and facilitate objective interpretation of variations in their morphology. These techniques may be used to investigate disorders involving cutaneous structures that are difficult to visualize by means of traditional microscopy.

Original languageEnglish
Pages (from-to)453-458
Number of pages6
JournalJournal of Cutaneous Pathology
Volume29
Issue number8
DOIs
StatePublished - Sep 2002

Fingerprint

Three-Dimensional Imaging
Photons
Confocal Microscopy
Microscopy
Mucous Membrane
Skin
Software
Biopsy
Blood Vessels
Rhodamines
Hair Follicle
Fluorescein
Lectins
Antibodies

ASJC Scopus subject areas

  • Dermatology
  • Pathology and Forensic Medicine

Cite this

Three-dimensional imaging of human skin and mucosa by two-photon laser scanning microscopy. / Malone, Janine C.; Hood, Antoinette F.; Conley, Tameka; Nürnberger, Jens; Baldridge, Lee Ann; Clendenon, Jeffrey L.; Dunn, Kenneth; Phillips, Carrie.

In: Journal of Cutaneous Pathology, Vol. 29, No. 8, 09.2002, p. 453-458.

Research output: Contribution to journalArticle

Malone, Janine C. ; Hood, Antoinette F. ; Conley, Tameka ; Nürnberger, Jens ; Baldridge, Lee Ann ; Clendenon, Jeffrey L. ; Dunn, Kenneth ; Phillips, Carrie. / Three-dimensional imaging of human skin and mucosa by two-photon laser scanning microscopy. In: Journal of Cutaneous Pathology. 2002 ; Vol. 29, No. 8. pp. 453-458.
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