Three-dimensional structures of pathogenic and saprophytic leptospira species revealed by cryo-electron tomography

Gianmarco Raddi, Dustin R. Morado, Jie Yan, David A. Haake, X. Frank Yang, Jun Liu

Research output: Contribution to journalArticle

44 Scopus citations

Abstract

Leptospira interrogans is the primary causative agent of the most widespread zoonotic disease, leptospirosis. An in-depth structural characterization of L. interrogans is needed to understand its biology and pathogenesis. In this study, cryo-electron tomography (cryo-ET) was used to compare pathogenic and saprophytic species and examine the unique morphological features of this group of bacteria. Specifically, our study revealed a structural difference between the cell envelopes of L. interrogans and Leptospira biflexa involving variations in the lipopolysaccharide (LPS) layer. Through cryo-ET and subvolume averaging, we determined the first three-dimensional (3-D) structure of the flagellar motor of leptospira, with novel features in the flagellar C ring, export apparatus, and stator. Together with direct visualization of chemoreceptor arrays, DNA packing, periplasmic filaments, spherical cytoplasmic bodies, and a unique " cap" at the cell end, this report provides structural insights into these fascinating Leptospira species.

Original languageEnglish (US)
Pages (from-to)1299-1306
Number of pages8
JournalJournal of bacteriology
Volume194
Issue number6
DOIs
StatePublished - Mar 1 2012

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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