Three-dimensional structures of pathogenic and saprophytic leptospira species revealed by cryo-electron tomography

Gianmarco Raddi, Dustin R. Morado, Jie Yan, David A. Haake, X. Yang, Jun Liu

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Leptospira interrogans is the primary causative agent of the most widespread zoonotic disease, leptospirosis. An in-depth structural characterization of L. interrogans is needed to understand its biology and pathogenesis. In this study, cryo-electron tomography (cryo-ET) was used to compare pathogenic and saprophytic species and examine the unique morphological features of this group of bacteria. Specifically, our study revealed a structural difference between the cell envelopes of L. interrogans and Leptospira biflexa involving variations in the lipopolysaccharide (LPS) layer. Through cryo-ET and subvolume averaging, we determined the first three-dimensional (3-D) structure of the flagellar motor of leptospira, with novel features in the flagellar C ring, export apparatus, and stator. Together with direct visualization of chemoreceptor arrays, DNA packing, periplasmic filaments, spherical cytoplasmic bodies, and a unique " cap" at the cell end, this report provides structural insights into these fascinating Leptospira species.

Original languageEnglish
Pages (from-to)1299-1306
Number of pages8
JournalJournal of Bacteriology
Volume194
Issue number6
DOIs
StatePublished - Mar 2012

Fingerprint

Electron Microscope Tomography
Leptospira interrogans
Leptospira
Leptospirosis
Zoonoses
Oligonucleotide Array Sequence Analysis
Cytoskeleton
Lipopolysaccharides
Bacteria

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Three-dimensional structures of pathogenic and saprophytic leptospira species revealed by cryo-electron tomography. / Raddi, Gianmarco; Morado, Dustin R.; Yan, Jie; Haake, David A.; Yang, X.; Liu, Jun.

In: Journal of Bacteriology, Vol. 194, No. 6, 03.2012, p. 1299-1306.

Research output: Contribution to journalArticle

Raddi, Gianmarco ; Morado, Dustin R. ; Yan, Jie ; Haake, David A. ; Yang, X. ; Liu, Jun. / Three-dimensional structures of pathogenic and saprophytic leptospira species revealed by cryo-electron tomography. In: Journal of Bacteriology. 2012 ; Vol. 194, No. 6. pp. 1299-1306.
@article{b4756f5ceb1f4bf1ad32de36341c75dd,
title = "Three-dimensional structures of pathogenic and saprophytic leptospira species revealed by cryo-electron tomography",
abstract = "Leptospira interrogans is the primary causative agent of the most widespread zoonotic disease, leptospirosis. An in-depth structural characterization of L. interrogans is needed to understand its biology and pathogenesis. In this study, cryo-electron tomography (cryo-ET) was used to compare pathogenic and saprophytic species and examine the unique morphological features of this group of bacteria. Specifically, our study revealed a structural difference between the cell envelopes of L. interrogans and Leptospira biflexa involving variations in the lipopolysaccharide (LPS) layer. Through cryo-ET and subvolume averaging, we determined the first three-dimensional (3-D) structure of the flagellar motor of leptospira, with novel features in the flagellar C ring, export apparatus, and stator. Together with direct visualization of chemoreceptor arrays, DNA packing, periplasmic filaments, spherical cytoplasmic bodies, and a unique {"} cap{"} at the cell end, this report provides structural insights into these fascinating Leptospira species.",
author = "Gianmarco Raddi and Morado, {Dustin R.} and Jie Yan and Haake, {David A.} and X. Yang and Jun Liu",
year = "2012",
month = "3",
doi = "10.1128/JB.06474-11",
language = "English",
volume = "194",
pages = "1299--1306",
journal = "Journal of Bacteriology",
issn = "0021-9193",
publisher = "American Society for Microbiology",
number = "6",

}

TY - JOUR

T1 - Three-dimensional structures of pathogenic and saprophytic leptospira species revealed by cryo-electron tomography

AU - Raddi, Gianmarco

AU - Morado, Dustin R.

AU - Yan, Jie

AU - Haake, David A.

AU - Yang, X.

AU - Liu, Jun

PY - 2012/3

Y1 - 2012/3

N2 - Leptospira interrogans is the primary causative agent of the most widespread zoonotic disease, leptospirosis. An in-depth structural characterization of L. interrogans is needed to understand its biology and pathogenesis. In this study, cryo-electron tomography (cryo-ET) was used to compare pathogenic and saprophytic species and examine the unique morphological features of this group of bacteria. Specifically, our study revealed a structural difference between the cell envelopes of L. interrogans and Leptospira biflexa involving variations in the lipopolysaccharide (LPS) layer. Through cryo-ET and subvolume averaging, we determined the first three-dimensional (3-D) structure of the flagellar motor of leptospira, with novel features in the flagellar C ring, export apparatus, and stator. Together with direct visualization of chemoreceptor arrays, DNA packing, periplasmic filaments, spherical cytoplasmic bodies, and a unique " cap" at the cell end, this report provides structural insights into these fascinating Leptospira species.

AB - Leptospira interrogans is the primary causative agent of the most widespread zoonotic disease, leptospirosis. An in-depth structural characterization of L. interrogans is needed to understand its biology and pathogenesis. In this study, cryo-electron tomography (cryo-ET) was used to compare pathogenic and saprophytic species and examine the unique morphological features of this group of bacteria. Specifically, our study revealed a structural difference between the cell envelopes of L. interrogans and Leptospira biflexa involving variations in the lipopolysaccharide (LPS) layer. Through cryo-ET and subvolume averaging, we determined the first three-dimensional (3-D) structure of the flagellar motor of leptospira, with novel features in the flagellar C ring, export apparatus, and stator. Together with direct visualization of chemoreceptor arrays, DNA packing, periplasmic filaments, spherical cytoplasmic bodies, and a unique " cap" at the cell end, this report provides structural insights into these fascinating Leptospira species.

UR - http://www.scopus.com/inward/record.url?scp=84863281646&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84863281646&partnerID=8YFLogxK

U2 - 10.1128/JB.06474-11

DO - 10.1128/JB.06474-11

M3 - Article

VL - 194

SP - 1299

EP - 1306

JO - Journal of Bacteriology

JF - Journal of Bacteriology

SN - 0021-9193

IS - 6

ER -