Thrombopoietin acts synergistically with LIF to maintain an undifferentiated state of embryonic stem cells homozygous for a Shp-2 deletion mutation

Xiaodong Xie, Rebecca Chan, Mervin Yoder

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Thrombopoietin (Tpo) and its receptor, c-mpl, are expressed in murine embryonic stem (ES) cells. ES cells are maintained in a pluripotent state by leukemia inhibitory factor (LIF) via activation of the Janus kinase (Jak)-STAT3 signaling pathway. Tpo, like LIF, activates STAT3. We report that Tpo increases the number of undifferentiated colonies derived from wild type or Shp-2 mutant (Shp-2Δ46-110) ES cells. Tpo plus LIF acted synergistically on the Shp-2Δ46-110 ES cells to maintain undifferentiated colonies but no evidence of synergism via Jak-STAT3 activation was detected. Collectively, these data suggest that Tpo can play a role in preventing ES cell differentiation via Jak-STAT3 activation and perhaps via novel pathways that are enhanced in the absence of functional Shp-2.

Original languageEnglish
Pages (from-to)361-364
Number of pages4
JournalFEBS Letters
Volume529
Issue number2-3
DOIs
StatePublished - Oct 9 2002

Fingerprint

Thrombopoietin
Leukemia Inhibitory Factor
Sequence Deletion
Embryonic Stem Cells
Stem cells
Janus Kinases
Chemical activation
Thrombopoietin Receptors
Cell Differentiation

Keywords

  • Differentiation
  • ES cell
  • LIF
  • Shp-2
  • STAT3
  • Tpo

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

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title = "Thrombopoietin acts synergistically with LIF to maintain an undifferentiated state of embryonic stem cells homozygous for a Shp-2 deletion mutation",
abstract = "Thrombopoietin (Tpo) and its receptor, c-mpl, are expressed in murine embryonic stem (ES) cells. ES cells are maintained in a pluripotent state by leukemia inhibitory factor (LIF) via activation of the Janus kinase (Jak)-STAT3 signaling pathway. Tpo, like LIF, activates STAT3. We report that Tpo increases the number of undifferentiated colonies derived from wild type or Shp-2 mutant (Shp-2Δ46-110) ES cells. Tpo plus LIF acted synergistically on the Shp-2Δ46-110 ES cells to maintain undifferentiated colonies but no evidence of synergism via Jak-STAT3 activation was detected. Collectively, these data suggest that Tpo can play a role in preventing ES cell differentiation via Jak-STAT3 activation and perhaps via novel pathways that are enhanced in the absence of functional Shp-2.",
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author = "Xiaodong Xie and Rebecca Chan and Mervin Yoder",
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T1 - Thrombopoietin acts synergistically with LIF to maintain an undifferentiated state of embryonic stem cells homozygous for a Shp-2 deletion mutation

AU - Xie, Xiaodong

AU - Chan, Rebecca

AU - Yoder, Mervin

PY - 2002/10/9

Y1 - 2002/10/9

N2 - Thrombopoietin (Tpo) and its receptor, c-mpl, are expressed in murine embryonic stem (ES) cells. ES cells are maintained in a pluripotent state by leukemia inhibitory factor (LIF) via activation of the Janus kinase (Jak)-STAT3 signaling pathway. Tpo, like LIF, activates STAT3. We report that Tpo increases the number of undifferentiated colonies derived from wild type or Shp-2 mutant (Shp-2Δ46-110) ES cells. Tpo plus LIF acted synergistically on the Shp-2Δ46-110 ES cells to maintain undifferentiated colonies but no evidence of synergism via Jak-STAT3 activation was detected. Collectively, these data suggest that Tpo can play a role in preventing ES cell differentiation via Jak-STAT3 activation and perhaps via novel pathways that are enhanced in the absence of functional Shp-2.

AB - Thrombopoietin (Tpo) and its receptor, c-mpl, are expressed in murine embryonic stem (ES) cells. ES cells are maintained in a pluripotent state by leukemia inhibitory factor (LIF) via activation of the Janus kinase (Jak)-STAT3 signaling pathway. Tpo, like LIF, activates STAT3. We report that Tpo increases the number of undifferentiated colonies derived from wild type or Shp-2 mutant (Shp-2Δ46-110) ES cells. Tpo plus LIF acted synergistically on the Shp-2Δ46-110 ES cells to maintain undifferentiated colonies but no evidence of synergism via Jak-STAT3 activation was detected. Collectively, these data suggest that Tpo can play a role in preventing ES cell differentiation via Jak-STAT3 activation and perhaps via novel pathways that are enhanced in the absence of functional Shp-2.

KW - Differentiation

KW - ES cell

KW - LIF

KW - Shp-2

KW - STAT3

KW - Tpo

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