Thymic stromal lymphopoietin amplifies the differentiation of alternatively activated macrophages

Hongwei Han, Mark B. Headley, Whitney Xu, Michael R. Comeau, Baohua Zhou, Steven F. Ziegler

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

The epithelial-derived cytokine thymic stromal lymphopoietin (TSLP) has been associated with the promotion of type 2 inflammation and the induction of allergic disease. In humans TSLP is elevated in the lungs of asthma patients and in the lesional skin of individuals with atopic dermatitis, whereas mice lacking TSLP responses are refractory to models of Th2-driven allergic disease. Although several cell types, including dendritic cells, basophils, and CD4 T cells, have been shown to respond to TSLP, its role in macrophage differentiation has not been studied. Type 2 cytokines (i.e., IL-4 and IL-13) can drive the differentiation of macrophages into alternatively activated macrophages (aaMfs, also referred to as M2 macrophages). This population of macrophages is associated with allergic inflammation. We therefore reasoned that TSLP/TSLPR signaling may be involved in the differentiation and activation of aaMfs during allergic airway inflammation. In this study, we report that TSLP changes the quiescent phenotype of pulmonary macrophages toward an aaMf phenotype during TSLP-induced airway inflammation. This differentiation of airway macrophages was IL-13-, but not IL-4-, dependent. Taken together, we demonstrate in this study that TSLP/TSLPR plays a significant role in the amplification of aaMF polarization and chemokine production, thereby contributing to allergic inflammation.

Original languageEnglish (US)
Pages (from-to)904-912
Number of pages9
JournalJournal of Immunology
Volume190
Issue number3
DOIs
StatePublished - Feb 1 2013

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Macrophages
Inflammation
Interleukin-13
Interleukin-4
Cytokines
Phenotype
Basophils
thymic stromal lymphopoietin
Alveolar Macrophages
Atopic Dermatitis
Chemokines
Dendritic Cells
Asthma
T-Lymphocytes
Lung
Skin
Population

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Thymic stromal lymphopoietin amplifies the differentiation of alternatively activated macrophages. / Han, Hongwei; Headley, Mark B.; Xu, Whitney; Comeau, Michael R.; Zhou, Baohua; Ziegler, Steven F.

In: Journal of Immunology, Vol. 190, No. 3, 01.02.2013, p. 904-912.

Research output: Contribution to journalArticle

Han, Hongwei ; Headley, Mark B. ; Xu, Whitney ; Comeau, Michael R. ; Zhou, Baohua ; Ziegler, Steven F. / Thymic stromal lymphopoietin amplifies the differentiation of alternatively activated macrophages. In: Journal of Immunology. 2013 ; Vol. 190, No. 3. pp. 904-912.
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