Thymic stromal lymphopoietin signaling in CD4+ T cells is required for TH2 memory

Qun Wang, Jianguang Du, Jingjing Zhu, Xiaowei Yang, Baohua Zhou

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Background Thymic stromal lymphopoietin (TSLP) is a key factor in the development of allergic asthma. Numbers of TH2 memory cells gradually increase in allergic patients with the progression of disease and persist in the lungs during remission, although the mechanism is not clear.

Objective We sought to define the role of TSLP in TH2 memory cell generation and maintenance in vivo.

Methods Adoptive transfer of wild-type and thymic stromal lymphopoietin receptor (TSLPR)-deficient ovalbumin-specific CD4+ T cells before TH2 sensitization was used to define T cell-specific TSLP effects. Atopic dermatitis and increased serum TSLP concentrations were induced by topical application of the vitamin D3 analog MC903. Memory cells in peripheral blood were monitored weekly with flow cytometry. Memory recall was tested after intranasal ovalbumin challenge.

Results TSLP signaling in CD4+ T cells is required for the generation/maintenance of memory cells after in vivo priming. TSLPR-deficient CD4+ T cells have no defects in proliferation but do not survive 1 week after sensitization, and increased TSLP expression during sensitization significantly increased the frequency of memory cells. Although in vitro-differentiated TSLPR-deficient TH2 cells develop into memory cells with equal efficiency to wild-type cells, the recall response to airway antigen challenge is impaired. Moreover, after antigen challenge of mice with established TH2 memory, TSLP signaling in CD4+ T cells significantly affects memory cell generation/maintenance from secondary effector cells.

Conclusion TSLP signaling in CD4+ T cells is required for not only TH2 memory cell formation in vivo but also the recall response of the memory cells to local antigen challenge.

Original languageEnglish
Pages (from-to)781-791.e3
JournalJournal of Allergy and Clinical Immunology
Volume135
Issue number3
DOIs
StatePublished - Mar 1 2015

Fingerprint

T-Lymphocytes
Maintenance
Ovalbumin
Antigens
thymic stromal lymphopoietin
CD4 Antigens
Adoptive Transfer
Cholecalciferol
Atopic Dermatitis
Disease Progression
Flow Cytometry
Asthma
Efficiency
Lung
Serum

Keywords

  • allergic airway inflammation
  • eosinophilia
  • memory recall
  • T2 memory
  • Thymic stromal lymphopoietin

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Thymic stromal lymphopoietin signaling in CD4+ T cells is required for TH2 memory. / Wang, Qun; Du, Jianguang; Zhu, Jingjing; Yang, Xiaowei; Zhou, Baohua.

In: Journal of Allergy and Clinical Immunology, Vol. 135, No. 3, 01.03.2015, p. 781-791.e3.

Research output: Contribution to journalArticle

Wang, Qun ; Du, Jianguang ; Zhu, Jingjing ; Yang, Xiaowei ; Zhou, Baohua. / Thymic stromal lymphopoietin signaling in CD4+ T cells is required for TH2 memory. In: Journal of Allergy and Clinical Immunology. 2015 ; Vol. 135, No. 3. pp. 781-791.e3.
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abstract = "Background Thymic stromal lymphopoietin (TSLP) is a key factor in the development of allergic asthma. Numbers of TH2 memory cells gradually increase in allergic patients with the progression of disease and persist in the lungs during remission, although the mechanism is not clear.Objective We sought to define the role of TSLP in TH2 memory cell generation and maintenance in vivo.Methods Adoptive transfer of wild-type and thymic stromal lymphopoietin receptor (TSLPR)-deficient ovalbumin-specific CD4+ T cells before TH2 sensitization was used to define T cell-specific TSLP effects. Atopic dermatitis and increased serum TSLP concentrations were induced by topical application of the vitamin D3 analog MC903. Memory cells in peripheral blood were monitored weekly with flow cytometry. Memory recall was tested after intranasal ovalbumin challenge.Results TSLP signaling in CD4+ T cells is required for the generation/maintenance of memory cells after in vivo priming. TSLPR-deficient CD4+ T cells have no defects in proliferation but do not survive 1 week after sensitization, and increased TSLP expression during sensitization significantly increased the frequency of memory cells. Although in vitro-differentiated TSLPR-deficient TH2 cells develop into memory cells with equal efficiency to wild-type cells, the recall response to airway antigen challenge is impaired. Moreover, after antigen challenge of mice with established TH2 memory, TSLP signaling in CD4+ T cells significantly affects memory cell generation/maintenance from secondary effector cells.Conclusion TSLP signaling in CD4+ T cells is required for not only TH2 memory cell formation in vivo but also the recall response of the memory cells to local antigen challenge.",
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N2 - Background Thymic stromal lymphopoietin (TSLP) is a key factor in the development of allergic asthma. Numbers of TH2 memory cells gradually increase in allergic patients with the progression of disease and persist in the lungs during remission, although the mechanism is not clear.Objective We sought to define the role of TSLP in TH2 memory cell generation and maintenance in vivo.Methods Adoptive transfer of wild-type and thymic stromal lymphopoietin receptor (TSLPR)-deficient ovalbumin-specific CD4+ T cells before TH2 sensitization was used to define T cell-specific TSLP effects. Atopic dermatitis and increased serum TSLP concentrations were induced by topical application of the vitamin D3 analog MC903. Memory cells in peripheral blood were monitored weekly with flow cytometry. Memory recall was tested after intranasal ovalbumin challenge.Results TSLP signaling in CD4+ T cells is required for the generation/maintenance of memory cells after in vivo priming. TSLPR-deficient CD4+ T cells have no defects in proliferation but do not survive 1 week after sensitization, and increased TSLP expression during sensitization significantly increased the frequency of memory cells. Although in vitro-differentiated TSLPR-deficient TH2 cells develop into memory cells with equal efficiency to wild-type cells, the recall response to airway antigen challenge is impaired. Moreover, after antigen challenge of mice with established TH2 memory, TSLP signaling in CD4+ T cells significantly affects memory cell generation/maintenance from secondary effector cells.Conclusion TSLP signaling in CD4+ T cells is required for not only TH2 memory cell formation in vivo but also the recall response of the memory cells to local antigen challenge.

AB - Background Thymic stromal lymphopoietin (TSLP) is a key factor in the development of allergic asthma. Numbers of TH2 memory cells gradually increase in allergic patients with the progression of disease and persist in the lungs during remission, although the mechanism is not clear.Objective We sought to define the role of TSLP in TH2 memory cell generation and maintenance in vivo.Methods Adoptive transfer of wild-type and thymic stromal lymphopoietin receptor (TSLPR)-deficient ovalbumin-specific CD4+ T cells before TH2 sensitization was used to define T cell-specific TSLP effects. Atopic dermatitis and increased serum TSLP concentrations were induced by topical application of the vitamin D3 analog MC903. Memory cells in peripheral blood were monitored weekly with flow cytometry. Memory recall was tested after intranasal ovalbumin challenge.Results TSLP signaling in CD4+ T cells is required for the generation/maintenance of memory cells after in vivo priming. TSLPR-deficient CD4+ T cells have no defects in proliferation but do not survive 1 week after sensitization, and increased TSLP expression during sensitization significantly increased the frequency of memory cells. Although in vitro-differentiated TSLPR-deficient TH2 cells develop into memory cells with equal efficiency to wild-type cells, the recall response to airway antigen challenge is impaired. Moreover, after antigen challenge of mice with established TH2 memory, TSLP signaling in CD4+ T cells significantly affects memory cell generation/maintenance from secondary effector cells.Conclusion TSLP signaling in CD4+ T cells is required for not only TH2 memory cell formation in vivo but also the recall response of the memory cells to local antigen challenge.

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