Time-dependent reduction of acetylcholine-induced relaxation in corpus cavernosum of cholestatic rats: Role of nitric oxide and cyclooxygenase pathway

Mehdi Dehghani, Hamed Sadeghipour, Hamed Shafaroodi, Hooman Honar, Kiarash Riazi, Mohammad Reza Ebrahimkhani, Amir Reza Hajrasouliha, Sina Tavakoli, Ahmad Reza Dehpour

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

The endothelium-dependent relaxation of corpus cavernosum smooth muscle and the roles of nitric oxide (NO) and arachidonic acid products of cyclooxygenase were investigated in non-operated, SHAM-operated, and bile duct-ligated rats. We further investigated the time-dependent alterations of corpus cavernosum relaxation in 2-, 7-, and 14-day bile duct-ligated animals. Acetylcholine produced concentration-dependent relaxation in phenylephrine-precontracted strips of corpus cavernosum. A significant reduction in the acetylcholine- induced relaxation was observed 2 days after bile duct ligation, and a greater reduction was observed on subsequent days. Incubation with 20 μM indomethacin reduced the acetylcholine-induced relaxation of the corpus cavernosum of unoperated rats while it had no effect in the corpus cavernosum of bile duct-ligated rats. Chronic treatment with Nω-Nitro-L-Arginine Methyl Ester (L-NAME, 3 mg/kg/day, intraperitoneally) reduced the relaxation responses in the unoperated group while it had no effect in the bile duct-ligated group. These results show that acetylcholine-induced corporal relaxation is impaired in cholestatic rats, and this may be related to deficient nitric oxide production by the endothelium. The involvement of prostaglandins in this impairment seems unlikely.

Original languageEnglish (US)
Pages (from-to)181-187
Number of pages7
JournalEuropean Journal of Pharmacology
Volume496
Issue number1
DOIs
StatePublished - Aug 2 2004

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Keywords

  • (Rat)
  • Acetylcholine
  • Cholestasis
  • Corpus cavernosum relaxation
  • Indomethacin
  • Nitric oxide

ASJC Scopus subject areas

  • Pharmacology

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